Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies
AbstractQuality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis. A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis. The existence of polysaccharides was also examined on a gel permeation chromatography column. The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples. The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%–0.21%, and 53.49%–58.2%, respectively. The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Chau, S.-L.; Huang, Z.-B.; Song, Y.-G.; Yue, R.-Q.; Ho, A.; Lin, C.-Z.; Huang, W.-H.; Han, Q.-B. Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies. Molecules 2016, 21, 1092.
Chau S-L, Huang Z-B, Song Y-G, Yue R-Q, Ho A, Lin C-Z, Huang W-H, Han Q-B. Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies. Molecules. 2016; 21(8):1092.Chicago/Turabian Style
Chau, Siu-Leung; Huang, Zhi-Bing; Song, Yan-Gang; Yue, Rui-Qi; Ho, Alan; Lin, Chao-Zhan; Huang, Wen-Hua; Han, Quan-Bin. 2016. "Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies." Molecules 21, no. 8: 1092.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.