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Molecules 2016, 21(8), 1092; doi:10.3390/molecules21081092

Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies

1
School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
2
State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, Nanchang 330047, China
3
Limin Pharmaceutical Factory, Livzon Group, Shaoguang 512028, China
4
Institute of Clinical Pharmacology, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China
These authors contribute equally to this paper.
*
Authors to whom correspondence should be addressed.
Academic Editor: Thomas Efferth
Received: 21 July 2016 / Revised: 11 August 2016 / Accepted: 15 August 2016 / Published: 19 August 2016
(This article belongs to the Collection Herbal Medicine Research)
View Full-Text   |   Download PDF [2205 KB, uploaded 19 August 2016]   |  

Abstract

Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis. A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis. The existence of polysaccharides was also examined on a gel permeation chromatography column. The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples. The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%–0.21%, and 53.49%–58.2%, respectively. The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections. View Full-Text
Keywords: Shenqi Fuzheng injection; Chinese medicine formula; quantitative analysis; chemical profile Shenqi Fuzheng injection; Chinese medicine formula; quantitative analysis; chemical profile
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Chau, S.-L.; Huang, Z.-B.; Song, Y.-G.; Yue, R.-Q.; Ho, A.; Lin, C.-Z.; Huang, W.-H.; Han, Q.-B. Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies. Molecules 2016, 21, 1092.

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