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Molecules 2016, 21(6), 736; doi:10.3390/molecules21060736

Solid Phase Formylation of N-Terminus Peptides

1
CROM, Istituto Nazionale Tumori “Fondazione G. Pascale”—IRCCS, 80131 Napoli, Italy
2
Inbios Srl, via Pietro Castellino 111, 80131 Napoli, Italy
3
Molecular Biology and Viral Oncology Unit, Research Department, Istituto Nazionale Tumori “Fondazione G.Pascale”—IRCCS, 80131 Napoli, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Alessandro Palmieri
Received: 26 April 2016 / Revised: 27 May 2016 / Accepted: 1 June 2016 / Published: 4 June 2016
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Abstract

Formylation of amino groups is a critical reaction involved in several biological processes including post-translational modification of histones. The addition of a formyl group (CHO) to the N-terminal end of a peptide chain generates biologically active molecules. N-formyl-peptides can be produced by different methods. We performed the N-formylation of two chemotactic hexapetides, Met1-Leu2-Lys3-Leu4-Ile5-Val6 and Met1-Met2-Tyr3-Ala4-Leu5-Phe6, carrying out the reaction directly on peptidyl-resin following pre-activation of formic acid with N,N-dicyclohexylcarbodiimmide (DCC) in liquid phase. The overnight incubation at 4 °C resulted in a significant increase in production yields of formylated peptides compared to the reaction performed at room temperature. The method is consistently effective, rapid, and inexpensive. Moreover, the synthetic strategy can be applied for the formylation of all primary amines at N-terminus of peptide chains or amino groups of lysine side-chains in solid phase. View Full-Text
Keywords: formylation; peptide; solid phase synthesis; amines formylation; peptide; solid phase synthesis; amines
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Tornesello, A.L.; Sanseverino, M.; Buonaguro, F.M. Solid Phase Formylation of N-Terminus Peptides. Molecules 2016, 21, 736.

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