Enantioselective Separation of 4,8-DHT and Phytotoxicity of the Enantiomers on Various Plant Species
Abstract
:1. Introduction
2. Results and Discussion
2.1. Separation of 4,8-DHT Enantiomers
2.2. Effects of Rac-4,8-DHT, S-(+)-Isosclerone, and R-(−)-Regiolone on Germination of the Tested Plants
2.3. Effects of Rac-4,8-DHT, S-(+)-Isosclerone, and R-(−)-Regiolone on Seedlings Growth of the Tested Plants
3. Materials and Methods
3.1. Chemicals, Plant Materials and Reagents
3.2. Chromatographic Separation and Analysis
3.3. Effects of 4,8-DHT on Seed Germination
3.4. Effects of 4,8-DHT on Seedling Growth
3.5. Statistic Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
4,8-DHT | 4,8-dihydroxy-1-tetralone |
HPLC | high-performance liquid chromatography |
CSP | chiral stationary phase |
AA | acetic acid |
SD | standard deviation |
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Plant Species | Forms | Germination Vigor | Germination Rate | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Control | 1 mM | 2 mM | 3 mM | 4 mM | Control | 1 mM | 2 mM | 3 mM | 4 mM | ||
Lettuce | rac | 75 ± 2 | 76 ± 1 | 83 ± 1 a | 69 ± 2 a | 60 ± 1 a | 85 ± 1 | 86 ± 1 | 94 ± 1 a | 83 ± 2 a | 79 ± 1 a |
S | 76 ± 2 | 85 ± 2 a | 65 ± 2 a,b | 57 ± 2 a,b | 87 ± 1 | 96 ± 1 a | 82 ± 2 a | 62 ± 1 a,b | |||
R | 76 ± 2 | 82 ± 2 a | 69 ± 2 a | 63 ± 2 a | 86 ± 2 | 92 ± 1 a | 83 ± 2 a | 80 ± 1 a | |||
Cucumber | rac | 83 ± 1 | 85 ± 1 a | 86 ± 2 a | 90 ± 2 a | 81 ± 2 | 87 ± 2 | 88 ± 2 | 90 ± 1 a | 93 ± 2 a | 82 ± 2 a |
S | 86 ± 2 a | 87 ± 2 a | 90 ± 2 a | 79 ± 2 a | 89 ± 1 | 90 ± 1 a | 93 ± 2 a | 80 ± 2 a,b | |||
R | 84 ± 2 | 86 ± 1 a | 89 ± 1 a | 81 ± 1 | 88 ± 2 | 88 ± 1 | 91 ± 1 a,b | 83 ± 1 a | |||
Radish | rac | 82 ± 1 | 83 ± 1 | 83 ± 1 | 86 ± 2 a | 79 ± 1 a | 88 ± 2 | 87 ± 2 | 89 ± 1 | 91 ± 1 | 83 ± 2 a |
S | 84 ± 1 a | 84 ± 2 a | 89 ± 1 a,b | 74 ± 1 a,b | 88 ± 2 | 90 ± 1 | 93 ± 1 a,b | 77 ± 2 a,b | |||
R | 82 ± 1 | 83 ± 1 | 85 ± 1 a | 80 ± 2 a | 88 ± 1 | 90 ± 1 | 90 ± 2 | 82 ± 2 a | |||
Onion | rac | 40 ± 4 | 63 ± 3 a,b | 36 ± 3 | 34 ± 2 a | 32 ± 1 a | 45 ± 4 | 68 ± 1 a,b | 43 ± 1 | 42 ± 1 a | 40 ± 1 a |
S | 67 ± 4 a,b | 33 ± 2 a | 32 ± 2 a | 30 ± 1 a | 69 ± 3 a,b | 40 ± 1 a,b | 39 ± 1 a | 33 ± 1 a,b | |||
R | 59 ± 2 ab | 36 ± 2 a | 34 ± 2 a | 33 ± 2 a | 63 ± 1 a | 43 ± 1 | 42 ± 1 a | 40 ± 1 a | |||
Wheat | rac | 68 ± 2 | 71 ± 2 | 64 ± 2 | 38 ± 5 a | 27 ± 2 a | 80 ± 2 | 83 ± 2 | 75 ± 4 a | 43 ± 5 a | 27 ± 2 a |
S | 74 ± 3a | 58 ± 3 a,b | 35 ± 4 a | 24 ± 3 a | 85 ± 1 a | 65 ± 1 a,b | 40 ± 4 a | 25 ± 1 a | |||
R | 70 ± 1 | 65 ± 1 | 39 ± 3 a | 28 ± 3 a | 83 ± 1 | 79 ± 3 | 50 ± 4 a,b | 29 ± 1 a |
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Yang, L.; Ma, X.-Y.; Ruan, X.; Jiang, D.-A.; Pan, C.-D.; Wang, Q. Enantioselective Separation of 4,8-DHT and Phytotoxicity of the Enantiomers on Various Plant Species. Molecules 2016, 21, 528. https://doi.org/10.3390/molecules21040528
Yang L, Ma X-Y, Ruan X, Jiang D-A, Pan C-D, Wang Q. Enantioselective Separation of 4,8-DHT and Phytotoxicity of the Enantiomers on Various Plant Species. Molecules. 2016; 21(4):528. https://doi.org/10.3390/molecules21040528
Chicago/Turabian StyleYang, Li, Xiao-Yan Ma, Xiao Ruan, De-An Jiang, Cun-De Pan, and Qiang Wang. 2016. "Enantioselective Separation of 4,8-DHT and Phytotoxicity of the Enantiomers on Various Plant Species" Molecules 21, no. 4: 528. https://doi.org/10.3390/molecules21040528