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Molecules 2016, 21(4), 474; doi:10.3390/molecules21040474

The Powdering Process with a Set of Ceramic Mills for Green Tea Promoted Catechin Extraction and the ROS Inhibition Effect

1
Division of Molecular Cell Biology, Core Research Facilities for Basic Science, The Jikei University School of Medicine, Tokyo 105-8461, Japan
2
Health and Environment Systems Division, Consumer Electronics Company, Sharp Corporation, Osaka 581-8585, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Rafael Llorach and Pedro Mena
Received: 3 February 2016 / Revised: 22 March 2016 / Accepted: 1 April 2016 / Published: 11 April 2016
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Abstract

For serving green tea, there are two prominent methods: steeping the leaf or the powdered leaf (matcha style) in hot water. The purpose of the present study was to reveal chemical and functional differences before and after the powdering process of green tea leaf, since powdered green tea may contribute to expanding the functionality because of the different ingesting style. In this study, we revealed that the powdering process with a ceramic mill and stirring in hot water increased the average extracted concentration of epigallocatechin gallate (EGCG) by more than three times compared with that in leaf tea using high-performance liquid chromatography (HPLC) and liquid chromatography–tandem mass Spectrometry (LC-MS/MS) analyses. Moreover, powdered green tea has a higher inhibition effect of reactive oxygen species (ROS) production in vitro compared with the same amount of leaf tea. Our data suggest that powdered green tea might have a different function from leaf tea due to the higher catechin contents and particles. View Full-Text
Keywords: green tea; matcha; catechin; polyphenol; powdering process; mill; ROS green tea; matcha; catechin; polyphenol; powdering process; mill; ROS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fujioka, K.; Iwamoto, T.; Shima, H.; Tomaru, K.; Saito, H.; Ohtsuka, M.; Yoshidome, A.; Kawamura, Y.; Manome, Y. The Powdering Process with a Set of Ceramic Mills for Green Tea Promoted Catechin Extraction and the ROS Inhibition Effect. Molecules 2016, 21, 474.

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