Encapsulation of Beetroot Pomace Extract: RSM Optimization, Storage and Gastrointestinal Stability
AbstractOne of the great problems in food production are surplus by-products, usually utilized for feeding animals and for preparation of dietary fibre or biofuel. These products represent potential sources of bioactive antioxidants and colour-giving compounds which could be used in the pharmaceutical industry and as food additives. In the present study beetroot pomace extract was encapsulated in soy protein by a freeze drying method. Process parameters (core: wall ratio, extract concentration and mixing time) were optimized using response surface methodology (RSM) in order to obtain the optimum encapsulate (OE) with the highest polyphenol encapsulation efficiency (EE) and radical scavenging activity on DPPH radicals (SA). Using the calculated optimum conditions, the EE (86.14%) and SA (1668.37 μmol Trolox equivalents/100 g) of OE did not differ significantly (p < 0.05) from the predicted ones. The contents of total polyphenols (326.51 mg GAE/100 g), flavonoids (10.23 mg RE/100 g), and betalains (60.52 mg betanin/100 g and 61.33 mg vulgaxanthin-I/100 g), individual content of phenolic compounds and betalains by HPLC, and the ability to reduce Fe3+ ions, i.e., reducing power (394.95 μmol Trolox equivalents/100 g) of OE were determined as well. During three months of storage at room temperature, polyphenol retention was much higher (76.67%) than for betalain pigments, betacyanins (17.77%) and betaxanthins (17.72%). In vitro digestion and release of phenolics from OE showed higher release rate in simulated intestinal fluid than in gastric fluid. These results suggest encapsulation as a contemporary method for valorisation of sensitive bioactive compounds from food industry by-products. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Tumbas Šaponjac, V.; Čanadanović-Brunet, J.; Ćetković, G.; Jakišić, M.; Djilas, S.; Vulić, J.; Stajčić, S. Encapsulation of Beetroot Pomace Extract: RSM Optimization, Storage and Gastrointestinal Stability. Molecules 2016, 21, 584.
Tumbas Šaponjac V, Čanadanović-Brunet J, Ćetković G, Jakišić M, Djilas S, Vulić J, Stajčić S. Encapsulation of Beetroot Pomace Extract: RSM Optimization, Storage and Gastrointestinal Stability. Molecules. 2016; 21(5):584.Chicago/Turabian Style
Tumbas Šaponjac, Vesna; Čanadanović-Brunet, Jasna; Ćetković, Gordana; Jakišić, Mirjana; Djilas, Sonja; Vulić, Jelena; Stajčić, Slađana. 2016. "Encapsulation of Beetroot Pomace Extract: RSM Optimization, Storage and Gastrointestinal Stability." Molecules 21, no. 5: 584.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.