Interactions of Bovine Serum Albumin with Anti-Cancer Compounds Using a ProteOn XPR36 Array Biosensor and Molecular Docking
AbstractThe aim of the work was to determine the interactions of a set of anti-cancer compounds with bovine serum albumin (BSA) using a ProteOn XPR36 array biosensor and molecular docking studies. The results revealed that a total of six anti-cancer compounds: gallic acid, doxorubicin, acteoside, salvianolic acid B, echinacoside, and vincristine were able to reversibly bind to the immobilized BSA. The sensorgrams of these six compounds were globally fit to a Langmuir 1:1 interaction model for binding kinetics analysis. There were significant differences in their affinity for BSA, with doxorubicin, the weakest binding compound having 1000-fold less affinity than salvianolic acid B, the strongest binding compound. However, compounds with a similar KD often exhibited markedly different kinetics due to the differences in ka and kd. Molecular docking experiments demonstrated that acteoside was partially located within sub-domain IIA of BSA, whereas gallic acid bound to BSA deep within its sub-domain IIIA. In addition, the interactions between these compounds and BSA were dominated by hydrophobic forces and hydrogen bonds. Understanding the detailed information of these anti-cancer compounds can provide important insights into optimizing the interactions and activity of potential compounds during drug development. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 3440 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Zhang, L.; Cai, Q.-Y.; Cai, Z.-X.; Fang, Y.; Zheng, C.-S.; Wang, L.-L.; Lin, S.; Chen, D.-X.; Peng, J. Interactions of Bovine Serum Albumin with Anti-Cancer Compounds Using a ProteOn XPR36 Array Biosensor and Molecular Docking. Molecules 2016, 21, 1706.
Zhang L, Cai Q-Y, Cai Z-X, Fang Y, Zheng C-S, Wang L-L, Lin S, Chen D-X, Peng J. Interactions of Bovine Serum Albumin with Anti-Cancer Compounds Using a ProteOn XPR36 Array Biosensor and Molecular Docking. Molecules. 2016; 21(12):1706.Chicago/Turabian Style
Zhang, Ling; Cai, Qiao-Yan; Cai, Zhi-Xiong; Fang, Yi; Zheng, Chun-Song; Wang, Li-Li; Lin, Shan; Chen, Da-Xin; Peng, Jun. 2016. "Interactions of Bovine Serum Albumin with Anti-Cancer Compounds Using a ProteOn XPR36 Array Biosensor and Molecular Docking." Molecules 21, no. 12: 1706.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.