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Molecules 2016, 21(12), 1703; doi:10.3390/molecules21121703

Enhanced Stilbene Production and Excretion in Vitis vinifera cv Pinot Noir Hairy Root Cultures

1
Laboratoire de Biologie des Plantes et Innovation EA 3900, SFR Condorcet FR CNRS 3417, UFR des Sciences, Ilot des Poulies, Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France
2
Unité de Recherche Vignes et Vins de Champagne EA 4707, SFR Condorcet FR CNRS 3417, UFR des Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne, BP 1039, 51687 Reims Cedex 2, France
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 14 October 2016 / Revised: 2 December 2016 / Accepted: 7 December 2016 / Published: 10 December 2016
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Abstract

Stilbenes are defense molecules produced by grapevine in response to stresses including various elicitors and signal molecules. Together with their prominent role in planta, stilbenes have been the center of much attention in recent decades due to their pharmaceutical properties. With the aim of setting up a cost-effective and high purity production of resveratrol derivatives, hairy root lines were established from Vitis vinifera cv Pinot Noir 40024 to study the organ-specific production of various stilbenes. Biomass increase and stilbene production by roots were monitored during flask experiments. Although there was a constitutive production of stilbenes in roots, an induction of stilbene synthesis by methyl jasmonate (MeJA) after 18 days of growth led to further accumulation of ε-viniferin, δ-viniferin, resveratrol and piceid. The use of 100 µM MeJA after 18 days of culture in the presence of methyl-β-cyclodextrins (MCDs) improved production levels, which reached 1034µg/g fresh weight (FW) in roots and 165 mg/L in the extracellular medium, corresponding to five-and 570-foldincrease in comparison to control. Whereas a low level of stilbene excretion was measured in controls, addition of MeJA induced excretion of up to 37% of total stilbenes. The use of MCDs increased the excretion phenomenon even more, reaching up to 98%. Our results demonstrate the ability of grapevine hairy roots to produce various stilbenes. This production was significantly improved in response to elicitation by methyl jasmonate and/or MCDs. This supports the interest of using hairy roots as a potentially valuable system for producing resveratrol derivatives. View Full-Text
Keywords: Vitis vinifera; hairy roots; resveratrol; viniferins; methyl jasmonate; cyclodextrins Vitis vinifera; hairy roots; resveratrol; viniferins; methyl jasmonate; cyclodextrins
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MDPI and ACS Style

Tisserant, L.-P.; Aziz, A.; Jullian, N.; Jeandet, P.; Clément, C.; Courot, E.; Boitel-Conti, M. Enhanced Stilbene Production and Excretion in Vitis vinifera cv Pinot Noir Hairy Root Cultures. Molecules 2016, 21, 1703.

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