Agarose and Its Derivatives as Supports for Enzyme Immobilization
AbstractAgarose is a polysaccharide obtained from some seaweeds, with a quite particular structure that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically resistant, chemically and physically inert, and sharply hydrophilic. These features—that could be further improved by means of covalent cross-linking—render them particularly suitable for enzyme immobilization with a wide range of derivatization methods taking advantage of chemical modification of a fraction of the polymer hydroxyls. The main properties of the polymer are described here, followed by a review of cross-linking and derivatization methods. Some recent, innovative procedures to optimize the catalytic activity and operational stability of the obtained preparations are also described, together with multi-enzyme immobilized systems and the main guidelines to exploit their performances. View Full-Text
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Zucca, P.; Fernandez-Lafuente, R.; Sanjust, E. Agarose and Its Derivatives as Supports for Enzyme Immobilization. Molecules 2016, 21, 1577.
Zucca P, Fernandez-Lafuente R, Sanjust E. Agarose and Its Derivatives as Supports for Enzyme Immobilization. Molecules. 2016; 21(11):1577.Chicago/Turabian Style
Zucca, Paolo; Fernandez-Lafuente, Roberto; Sanjust, Enrico. 2016. "Agarose and Its Derivatives as Supports for Enzyme Immobilization." Molecules 21, no. 11: 1577.
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