Real-Time Detection of a Self-Replicating RNA Enzyme
AbstractA system was developed to detect the self-replication of an RNA enzyme in real time. The enzyme is an RNA ligase that undergoes exponential amplification at a constant temperature and can be made to operate in a ligand-dependent manner. The real-time system is based on a fluorimetric readout that directly couples the ligation event to an increase in florescence signal that can be monitored using standard instrumentation. The real-time system can also operate entirely with l-RNA, which is not susceptible to degradation by ribonucleases that are present in biological samples. The system is analogous to real-time PCR, but with the potential to detect small molecules, proteins, and other targets that can be recognized by a suitable aptamer. The ligand-dependent self-replication of RNA has potential applications in molecular diagnostics and biosensing that benefit from the rapid, precise, and real-time detection of various target molecules. View Full-Text
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Olea, C.; Joyce, G.F. Real-Time Detection of a Self-Replicating RNA Enzyme. Molecules 2016, 21, 1310.
Olea C, Joyce GF. Real-Time Detection of a Self-Replicating RNA Enzyme. Molecules. 2016; 21(10):1310.Chicago/Turabian Style
Olea, Charles; Joyce, Gerald F. 2016. "Real-Time Detection of a Self-Replicating RNA Enzyme." Molecules 21, no. 10: 1310.
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