Next Article in Journal
SimCP3—An Advanced Homologue of SimCP2 as a Solution-Processed Small Molecular Host Material for Blue Phosphorescence Organic Light-Emitting Diodes
Next Article in Special Issue
Accumulation of Stable Full-Length Circular Group I Intron RNAs during Heat-Shock
Previous Article in Journal
Nucleophilic Substitution on 2-Monosubstituted Quinoxalines Giving 2,3-Disubstituted Quinoxalines: Investigating the Effect of the 2-Substituent
Previous Article in Special Issue
Group I Intron Internal Guide Sequence Binding Strength as a Component of Ribozyme Network Formation
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(10), 1310; doi:10.3390/molecules21101310

Real-Time Detection of a Self-Replicating RNA Enzyme

Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Sabine Müller
Received: 6 September 2016 / Revised: 25 September 2016 / Accepted: 27 September 2016 / Published: 30 September 2016
(This article belongs to the Special Issue Ribozymes and RNA Catalysis)
View Full-Text   |   Download PDF [1215 KB, uploaded 30 September 2016]   |  

Abstract

A system was developed to detect the self-replication of an RNA enzyme in real time. The enzyme is an RNA ligase that undergoes exponential amplification at a constant temperature and can be made to operate in a ligand-dependent manner. The real-time system is based on a fluorimetric readout that directly couples the ligation event to an increase in florescence signal that can be monitored using standard instrumentation. The real-time system can also operate entirely with l-RNA, which is not susceptible to degradation by ribonucleases that are present in biological samples. The system is analogous to real-time PCR, but with the potential to detect small molecules, proteins, and other targets that can be recognized by a suitable aptamer. The ligand-dependent self-replication of RNA has potential applications in molecular diagnostics and biosensing that benefit from the rapid, precise, and real-time detection of various target molecules. View Full-Text
Keywords: aptazyme; exponential amplification; fluorescence detection; in vitro selection; RNA enzyme; RNA ligation; self-replication aptazyme; exponential amplification; fluorescence detection; in vitro selection; RNA enzyme; RNA ligation; self-replication
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Olea, C.; Joyce, G.F. Real-Time Detection of a Self-Replicating RNA Enzyme. Molecules 2016, 21, 1310.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top