Development of Sulfadiazine-Decorated PLGA Nanoparticles Loaded with 5-Fluorouracil and Cell Viability
AbstractThe aim of this work was to synthesize sulfadiazine-poly(lactide-co-glycolide) (SUL-PLGA) nanoparticles (NPs) for the efficient delivery of 5-fluorouracil to cancer cells. The SUL-PLGA conjugation was assessed using FTIR, 1H-NMR, 13C-NMR, elemental analysis and TG and DTA analysis. The SUL-PLGA NPs were characterized using transmission and scanning electron microscopy and dynamic light scattering. Additionally, the zeta potential, drug content, and in vitro 5-FU release were evaluated. We found that for the SUL-PLGA NPs, Dh = 114.0 nm, ZP = −32.1 mV and the encapsulation efficiency was 49%. The 5-FU was released for up to 7 days from the NPs. Cytotoxicity evaluations of 5-FU-loaded NPs (5-FU-SUL-PLGA and 5-FU-PLGA) on two cancer cell lines (Caco-2, A431) and two normal cell lines (fibroblast, osteoblast) were compared. Higher cytotoxicity of 5-FU-SUL-PLGA NPs were found to both cancer cell lines when compared to normal cell lines, demonstrating that the presence of SUL could significantly enhance the cytotoxicity of the 5-FU-SUL-PLGA NPs when compared with 5-FU-PLGA NPs. Thus, the development of 5-FU-SUL-PLGA NPs to cancer cells is a promising strategy for the 5-FU antitumor formulation in the future. View Full-Text
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Guimarães, P.P.G.; Oliveira, S.R.; de Castro Rodrigues, G.; Gontijo, S.M.L.; Lula, I.S.; Cortés, M.E.; Denadai, Â.M.L.; Sinisterra, R.D. Development of Sulfadiazine-Decorated PLGA Nanoparticles Loaded with 5-Fluorouracil and Cell Viability. Molecules 2015, 20, 879-899.
Guimarães PPG, Oliveira SR, de Castro Rodrigues G, Gontijo SML, Lula IS, Cortés ME, Denadai ÂML, Sinisterra RD. Development of Sulfadiazine-Decorated PLGA Nanoparticles Loaded with 5-Fluorouracil and Cell Viability. Molecules. 2015; 20(1):879-899.Chicago/Turabian Style
Guimarães, Pedro P.G.; Oliveira, Sheila R.; de Castro Rodrigues, Gabrielle; Gontijo, Savio M.L.; Lula, Ivana S.; Cortés, Maria E.; Denadai, Ângelo M.L.; Sinisterra, Rubén D. 2015. "Development of Sulfadiazine-Decorated PLGA Nanoparticles Loaded with 5-Fluorouracil and Cell Viability." Molecules 20, no. 1: 879-899.