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Molecules 2015, 20(1), 293-308; doi:10.3390/molecules20010293

Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway

1
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China
2
Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 12 November 2014 / Accepted: 19 December 2014 / Published: 24 December 2014
(This article belongs to the Section Medicinal Chemistry)
View Full-Text   |   Download PDF [1039 KB, uploaded 24 December 2014]   |  

Abstract

Oxidative stress plays an important role in Parkinson’s disease and other neurodegenerative disorders. Lycium barbarum polysaccharides (LBP), the main active ingredients extracted from the fruits of Lycium barbarum L., have been shown to be a potent antioxidant. In the present study, we investigated the protective effects, and the possible mechanism of action of LBP against 6-hydroxydopamine (6-OHDA)-induced apoptosis in PC12 cells. Our data demonstrated that LBP significantly reversed the 6-OHDA-induced decrease in cell viability, prevented 6-OHDA-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. Furthermore, LBP also slowed the accumulation of reactive oxygen species (ROS) and nitric oxide (NO), decreased the level of protein-bound 3-nitrotyrosine (3-NT) and intracellular free Ca2+, and inhibiting the overexpression of nuclear factor κB (NF-κB), neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS). These results demonstrate that LBP prevents 6-OHDA-induced apoptosis in PC12 cells, at least in part through the ROS-NO pathway. View Full-Text
Keywords: Parkinson’s disease; LBP; PC cells; apoptosis; reactive oxygen species; nitric oxide; mitochondrion Parkinson’s disease; LBP; PC cells; apoptosis; reactive oxygen species; nitric oxide; mitochondrion
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gao, K.; Liu, M.; Cao, J.; Yao, M.; Lu, Y.; Li, J.; Zhu, X.; Yang, Z.; Wen, A. Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway. Molecules 2015, 20, 293-308.

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