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Molecules 2014, 19(9), 15103-15115; doi:10.3390/molecules190915103

LC-ESI-MS/MS Analysis and Pharmacokinetics of Plantainoside D Isolated from Chirita longgangensis var. hongyao, a Potential Anti-Hypertensive Active Component in Rats

1
Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
2
Faculty of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
3
Department of Pharmacy, Hebei North University, Zhangjiakou 075000, Hebei, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 17 July 2014 / Revised: 4 September 2014 / Accepted: 4 September 2014 / Published: 22 September 2014
(This article belongs to the Collection Bioactive Compounds)
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Abstract

Plantainoside D (PD) is a potential anti-hypertensive active ingredient newly isolated from the dried plants of Chirita longgangensis var. hongyao. A sensitive and specific LC-ESI-MS/MS method was first developed and validated for the analysis of PD in rat plasma using genistein as the internal standard (IS). The plasma samples were pretreated with methanol-acetonitrile (50:50, v/v) to precipitate protein, and then chromatographed on a reverse-phase Agilent Zorbax XDB C18 column (50 mm × 2.1 mm, 3.5 μm). Gradient elution was utilized, with a mobile phase consisting of water and acetonitrile both containing 0.1% formic acid, and the flow rate was set at 0.50 mL/min. The analytes were monitored by tandem-mass spectrometry with negative electrospray ionization. The precursor/product transitions (m/z) in the negative ion mode were 639.2 → 160.9 Thomson (Th) and 268.9 → 158.9 Thomson (Th) for PD and IS, respectively. Linearity was achieved in the 0.10–200 ng/mL range, with a lower limit of quantification of 0.10 ng/mL. The precision and accuracy for both intra- and inter-day determination of the analyte were all within ±15%. The present method has been applied for pharmacokinetic study of PD after oral and intravenous administration in rats. The oral absolute bioavailability (F) of PD in rats was estimated to be 1.12% ± 0.46% with an elimination half-life (t1/2) value of 1.63 ± 0.19 h, suggesting its poor absorption and/or strong metabolism in vivo. View Full-Text
Keywords: Chirita longgangensis var. hongyao; plantainoside D; bioavailability; LC-ESI-MS/MS; rat Chirita longgangensis var. hongyao; plantainoside D; bioavailability; LC-ESI-MS/MS; rat
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MDPI and ACS Style

Wang, M.; Fu, S.; Zhang, X.; Li, J.; Gong, M.; Qiu, F. LC-ESI-MS/MS Analysis and Pharmacokinetics of Plantainoside D Isolated from Chirita longgangensis var. hongyao, a Potential Anti-Hypertensive Active Component in Rats. Molecules 2014, 19, 15103-15115.

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