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Molecules 2014, 19(10), 15374-15390; doi:10.3390/molecules191015374

Identification of Three Elicitins and a Galactan-Based Complex Polysaccharide from a Concentrated Culture Filtrate of Phytophthora infestans Efficient against Pectobacterium atrosepticum

1
INRA, UMR1349 IGEPP, Le Rheu-Cedex F-35653, France
2
CNRS-Université Pierre et Marie Curie, FR2424, Station Biologique de Roscoff, Roscoff-Cedex 29682, France
3
Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708, France
4
Agrocampus Ouest, UMR1349 IGEPP, Rennes F-35000, France
5
Université Européenne de Bretagne, Rennes F-35000, France
*
Authors to whom correspondence should be addressed.
Received: 29 July 2014 / Revised: 10 September 2014 / Accepted: 15 September 2014 / Published: 26 September 2014
(This article belongs to the Collection Bioactive Compounds)
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Abstract

The induction of plant immunity by Pathogen Associated Molecular Patterns (PAMPs) constitutes a powerful strategy for crop protection. PAMPs indeed induce general defense responses in plants and thus increase plant resistance to pathogens. Phytophthora infestans culture filtrates (CCFs) are known to induce defense responses and decrease the severity of soft rot due to Pectobacterium atrosepticum in potato tubers. The aim of this study was to identify and characterize the active compounds from P. infestans filtrate. The filtrate was fractionated by gel filtration, and the protection effects against P. atrosepticum and the ability to induce PAL activity were tested for each fraction. The fraction active in protection (F1) also induced PAL activity, as did the whole filtrate. Three elicitins (INF1, INF4 and INF5) were identified in F1b, subfraction of F1, by MALDI-TOF-MS and MS/MS analyses. However, deproteinized F1b still showed biological activity against the bacterium, revealing the presence of an additional active compound. GC-MS analyses of the deproteinized fraction highlighted the presence of a galactan-based complex polysaccharide. These experiments demonstrate that the biological activity of the CCF against P. atrosepticum results from a combined action of three elicitins and a complex polysaccharide, probably through the activation of general defense responses. View Full-Text
Keywords: elicitin; GC-MS; MALDI TOF; PAMPs-triggered immunity; phenylalanine ammonialyase; polysaccharide; plant defense responses; Solanum tuberosum elicitin; GC-MS; MALDI TOF; PAMPs-triggered immunity; phenylalanine ammonialyase; polysaccharide; plant defense responses; Solanum tuberosum
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Saubeau, G.; Gaillard, F.; Legentil, L.; Nugier-Chauvin, C.; Ferrières, V.; Andrivon, D.; Val, F. Identification of Three Elicitins and a Galactan-Based Complex Polysaccharide from a Concentrated Culture Filtrate of Phytophthora infestans Efficient against Pectobacterium atrosepticum. Molecules 2014, 19, 15374-15390.

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