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Molecules 2014, 19(9), 14567-14581; https://doi.org/10.3390/molecules190914567

Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract

1
Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju 690-756, Korea
2
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea
3
Faculty of Biotechnology, School of Applied Life Science, Jeju National University, Jeju 690-756, Korea
4
BRM Institute, Seoul 135-010, Korea
5
Department of Animal Biotechnology, School of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Korea
6
Department of Oral Biochemistry, Dental Science Research Institute, Chonnam National University, Gwangju 500-757, Korea
*
Author to whom correspondence should be addressed.
Received: 9 August 2014 / Accepted: 4 September 2014 / Published: 15 September 2014
(This article belongs to the Section Medicinal Chemistry)
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Abstract

This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The E. hirta ethanol extract (0.5 mg/mL) exhibited DPPH-scavenging activity of 61.19% ± 0.22%, while the positive control (0.5 mg/mL ascorbic acid) had 100% ± 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC50) was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 µg/mL E. hirta extract, and nitric oxide production was decreased significantly (p < 0.05). The extract also showed selective anticancer activity at a concentration of 100 µg/mL (p < 0.05). These results indicated that E. hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications. View Full-Text
Keywords: Euphorbia hirta; chemical composition; online HPLC-ABTS+• bioassay; antioxidant; anti-inflammatory; anticancer Euphorbia hirta; chemical composition; online HPLC-ABTS+• bioassay; antioxidant; anti-inflammatory; anticancer
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Sharma, N.; Samarakoon, K.W.; Gyawali, R.; Park, Y.-H.; Lee, S.-J.; Oh, S.J.; Lee, T.-H.; Jeong, D.K. Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract. Molecules 2014, 19, 14567-14581.

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