Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery
AbstractPreviously, we have reported the synthesis of a homochiral l-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]5 and [WR]5-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine. View Full-Text
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Shirazi, A.N.; Paquin, K.L.; Howlett, N.G.; Mandal, D.; Parang, K. Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery. Molecules 2014, 19, 13319-13331.
Shirazi AN, Paquin KL, Howlett NG, Mandal D, Parang K. Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery. Molecules. 2014; 19(9):13319-13331.Chicago/Turabian Style
Shirazi, Amir N.; Paquin, Karissa L.; Howlett, Niall G.; Mandal, Dindyal; Parang, Keykavous. 2014. "Cyclic Peptide-Capped Gold Nanoparticles for Enhanced siRNA Delivery." Molecules 19, no. 9: 13319-13331.