Next Article in Journal
Antifungal Plant Defensins: Mechanisms of Action and Production
Next Article in Special Issue
Allicin: Chemistry and Biological Properties
Previous Article in Journal
The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes
Previous Article in Special Issue
Cudarflavone B Provides Neuroprotection against Glutamate-Induced Mouse Hippocampal HT22 Cell Damage through the Nrf2 and PI3K/Akt Signaling Pathways
Article Menu

Export Article

Open AccessArticle
Molecules 2014, 19(8), 12258-12279; doi:10.3390/molecules190812258

Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems

1
Department of Genetics, Cancer Research Institute, Vlárska 7, 833 91 Bratislava, Slovakia
2
Department of Oncology, Cancer Research Institute, Vlárska 7, 833 91 Bratislava, Slovakia
3
Division of Bioorganic Chemistry, School of Pharmacy, Saarland University, Campus B 2.1, D-66123 Saarbruecken, Germany
4
CAPES Foundation, Ministry of Education of Brazil, DF 70040-020 Brasilia, Brazil
5
Department of Plant Physiology (BioIII), RWTH Aachen University, D-52056 Aachen, Germany
*
Authors to whom correspondence should be addressed.
Received: 13 June 2014 / Revised: 22 July 2014 / Accepted: 24 July 2014 / Published: 13 August 2014
View Full-Text   |   Download PDF [2882 KB, uploaded 13 August 2014]   |  

Abstract

Redox-modulating compounds derived from natural sources, such as redox active secondary metabolites, are currently of considerable interest in the field of chemoprevention, drug and phytoprotectant development. Unfortunately, the exact and occasionally even selective activity of such products, and the underlying (bio-)chemical causes thereof, are often only poorly understood. A combination of the nematode- and yeast-based assays provides a powerful platform to investigate a possible biological activity of a new compound and also to explore the “redox link” which may exist between its activity on the one side and its chemistry on the other. Here, we will demonstrate the usefulness of this platform for screening several selenium and tellurium compounds for their activity and action. We will also show how the nematode-based assay can be used to obtain information on compound uptake and distribution inside a multicellular organism, whilst the yeast-based system can be employed to explore possible intracellular mechanisms via chemogenetic screening and intracellular diagnostics. Whilst none of these simple and easy-to-use assays can ultimately substitute for in-depth studies in human cells and animals, these methods nonetheless provide a first glimpse on the possible biological activities of new compounds and offer direction for more complicated future investigations. They may also uncover some rather unpleasant biochemical actions of certain compounds, such as the ability of the trace element supplement selenite to induce DNA strand breaks. View Full-Text
Keywords: chemogenomic screening, intracellular thiolstat, intracellular diagnostics, redox modulation, nematodes, Saccharomyces cerevisiae, thiolstat chemogenomic screening, intracellular thiolstat, intracellular diagnostics, redox modulation, nematodes, Saccharomyces cerevisiae, thiolstat
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Mániková, D.; Letavayová, L.M.; Vlasáková, D.; Košík, P.; Estevam, E.C.; Nasim, M.J.; Gruhlke, M.; Slusarenko, A.; Burkholz, T.; Jacob, C.; Chovanec, M. Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems. Molecules 2014, 19, 12258-12279.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top