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Molecules 2014, 19(8), 11419-11439; doi:10.3390/molecules190811419
Article

Biocatalytic Behaviour of Immobilized Rhizopus oryzae Lipase in the 1,3-Selective Ethanolysis of Sunflower Oil to Obtain a Biofuel Similar to Biodiesel

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Received: 5 May 2014; in revised form: 22 July 2014 / Accepted: 22 July 2014 / Published: 4 August 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract: A new biofuel similar to biodiesel was obtained in the 1,3-selective transesterification reaction of sunflower oil with ethanol using as biocatalyst a Rhizopus oryzae lipase (ROL) immobilized on Sepiolite, an inorganic support. The studied lipase was a low cost powdered enzyme preparation, Biolipase-R, from Biocon-Spain, a multipurpose additive used in food industry. In this respect, it is developed a study to optimize the immobilization procedure of these lipases on Sepiolite. Covalent immobilization was achieved by the development of an inorganic-organic hybrid linker formed by a functionalized hydrocarbon chain with a pendant benzaldehyde, bonded to the AlPO4 support surface. Thus, the covalent immobilization of lipases on amorphous AlPO4/sepiolite (20/80 wt %) support was evaluated by using two different linkers (p-hydroxybenzaldehyde and benzylamine-terephthalic aldehyde, respectively). Besides, the catalytic behavior of lipases after physical adsorption on the demineralized sepiolite  was also evaluated. Obtained results indicated that covalent immobilization with the p-hydroxybenzaldehyde linker gave the best biocatalytic behavior. Thus, this covalently immobilized lipase showed a remarkable stability as well as an excellent capacity of reutilization (more than five successive reuses) without a significant loss of its initial catalytic activity. This could allow a more efficient fabrication of biodiesel minimizing the glycerol waste production.
Keywords: biodiesel; immobilization; Rhizopus oryzae lipase; Biolipase-R; amorphous AlPO4/sepiolite; demineralized sepiolite; selective transesterification; monoglyceride; Ecodiesel; sunflower oil biodiesel; immobilization; Rhizopus oryzae lipase; Biolipase-R; amorphous AlPO4/sepiolite; demineralized sepiolite; selective transesterification; monoglyceride; Ecodiesel; sunflower oil
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Luna, C.; Verdugo, C.; Sancho, E.D.; Luna, D.; Calero, J.; Posadillo, A.; Bautista, F.M.; Romero, A.A. Biocatalytic Behaviour of Immobilized Rhizopus oryzae Lipase in the 1,3-Selective Ethanolysis of Sunflower Oil to Obtain a Biofuel Similar to Biodiesel. Molecules 2014, 19, 11419-11439.

AMA Style

Luna C, Verdugo C, Sancho ED, Luna D, Calero J, Posadillo A, Bautista FM, Romero AA. Biocatalytic Behaviour of Immobilized Rhizopus oryzae Lipase in the 1,3-Selective Ethanolysis of Sunflower Oil to Obtain a Biofuel Similar to Biodiesel. Molecules. 2014; 19(8):11419-11439.

Chicago/Turabian Style

Luna, Carlos; Verdugo, Cristóbal; Sancho, Enrique D.; Luna, Diego; Calero, Juan; Posadillo, Alejandro; Bautista, Felipa M.; Romero, Antonio A. 2014. "Biocatalytic Behaviour of Immobilized Rhizopus oryzae Lipase in the 1,3-Selective Ethanolysis of Sunflower Oil to Obtain a Biofuel Similar to Biodiesel." Molecules 19, no. 8: 11419-11439.



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