Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization
AbstractWe report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w).
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Qian, Y.-C.; Chen, P.-C.; He, G.-J.; Huang, X.-J.; Xu, Z.-K. Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization. Molecules 2014, 19, 9850-9863.
Qian Y-C, Chen P-C, He G-J, Huang X-J, Xu Z-K. Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization. Molecules. 2014; 19(7):9850-9863.Chicago/Turabian Style
Qian, Yue-Cheng; Chen, Peng-Cheng; He, Gui-Jin; Huang, Xiao-Jun; Xu, Zhi-Kang. 2014. "Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization." Molecules 19, no. 7: 9850-9863.