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Molecules 2014, 19(7), 9850-9863; doi:10.3390/molecules19079850
Article

Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization

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*  and
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
* Author to whom correspondence should be addressed.
Received: 19 May 2014 / Revised: 23 June 2014 / Accepted: 24 June 2014 / Published: 8 July 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract

We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w).
Keywords: polyphosphazene hydrogel; swelling; lipase; entrapment; activity polyphosphazene hydrogel; swelling; lipase; entrapment; activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Qian, Y.-C.; Chen, P.-C.; He, G.-J.; Huang, X.-J.; Xu, Z.-K. Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization. Molecules 2014, 19, 9850-9863.

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