Next Article in Journal
T-Type Ca2+ Channel Blocker, KYS05090 Induces Autophagy and Apoptosis in A549 Cells through Inhibiting Glucose Uptake
Next Article in Special Issue
Nucleoside 2'-Deoxyribosyltransferase from Psychrophilic Bacterium Bacillus psychrosaccharolyticus — Preparation of an Immobilized Biocatalyst for the Enzymatic Synthesis of Therapeutic Nucleosides
Previous Article in Journal / Special Issue
Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis
Molecules 2014, 19(7), 9850-9863; doi:10.3390/molecules19079850

Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization

*  and
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
* Author to whom correspondence should be addressed.
Received: 19 May 2014 / Revised: 23 June 2014 / Accepted: 24 June 2014 / Published: 8 July 2014
(This article belongs to the Special Issue Enzyme Immobilization)
View Full-Text   |   Download PDF [490 KB, uploaded 8 July 2014]   |  


We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w).
Keywords: polyphosphazene hydrogel; swelling; lipase; entrapment; activity polyphosphazene hydrogel; swelling; lipase; entrapment; activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Qian, Y.-C.; Chen, P.-C.; He, G.-J.; Huang, X.-J.; Xu, Z.-K. Preparation of Polyphosphazene Hydrogels for Enzyme Immobilization. Molecules 2014, 19, 9850-9863.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert