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Molecules 2014, 19(7), 9864-9875; doi:10.3390/molecules19079864

T-Type Ca2+ Channel Blocker, KYS05090 Induces Autophagy and Apoptosis in A549 Cells through Inhibiting Glucose Uptake

1
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Korea
2
Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Korea
3
Department of Biomedical Science, College of Medical Science, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Korea
4
Research Institute for Basic Sciences and Department of Chemistry, College of Sciences, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Korea
5
Department of Molecular Biology, College of Pharmacy, Kyung Hee University, Seoul 130-701, Korea
*
Authors to whom correspondence should be addressed.
Received: 27 May 2014 / Revised: 27 June 2014 / Accepted: 3 July 2014 / Published: 8 July 2014
(This article belongs to the Section Medicinal Chemistry)
View Full-Text   |   Download PDF [384 KB, uploaded 11 July 2014]   |  

Abstract

It has been reported that [3-(1,1'-biphenyl-4-yl)-2-(1-methyl-5-dimethylamino-pentylamino)-3,4-dihydroquinazolin-4-yl]-N-benzylacetamide 2hydrochloride (KYS05090), a selective T-type Ca2+ channel blocker, reduces tumor volume and weight in the A549 xenograft model, but the molecular mechanism of cell death has not yet been elucidated. In this study, KYS05090 induced autophagy- and apoptosis-mediated cell death in human lung adenocarcinoma A549 cells. Although KYS05090 decreased intracellular Ca2+ levels, it was not directly related with KYS05090-induced cell death. In addition, KYS05090 generated intracellular reactive oxygen species (ROS) and reduced glucose uptake, and catalase and methyl pyruvate prevented KYS05090-induced cell death. These results indicate that KYS05090 can lead to autophagy and apoptosis in A549 cells through ROS generation by inhibiting glucose uptake. Our findings suggest that KYS05090 has potential chemotherapeutic value for the treatment of lung cancer. View Full-Text
Keywords: T-type Ca2+ channel; glucose; autophagy; apoptosis; reactive oxygen species T-type Ca2+ channel; glucose; autophagy; apoptosis; reactive oxygen species
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Rim, H.-K.; Cho, S.; Shin, D.-H.; Chung, K.-S.; Cho, Y.-W.; Choi, J.-H.; Lee, J.Y.; Lee, K.-T. T-Type Ca2+ Channel Blocker, KYS05090 Induces Autophagy and Apoptosis in A549 Cells through Inhibiting Glucose Uptake. Molecules 2014, 19, 9864-9875.

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