Molecules 2014, 19(7), 9838-9849; doi:10.3390/molecules19079838
Article

Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis

1email, 2email, 2email, 2email, 2email, 2email, 3,* email and 2,* email
Received: 21 May 2014; in revised form: 3 July 2014 / Accepted: 4 July 2014 / Published: 8 July 2014
(This article belongs to the Special Issue Enzyme Immobilization)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The immobilized thermophilic esterase from Archaeoglobus fulgidus was successfully constructed through the glutaraldehyde-mediated covalent coupling after its physical adsorption on a hydrophobic macroporous resin, Sepabeads EC-OD. Through 0.05% glutaraldehyde treatment, the prevention of enzyme leaching and the maintenance of catalytic activity could be simultaneously realized. Using the enzymatic ring-opening polymerization of ε-caprolactone as a model, effects of organic solvents and reaction temperature on the monomer conversion and product molecular weight were systematically investigated. After the optimization of reaction conditions, products were obtained with 100% monomer conversion and Mn values lower than 1010 g/mol. Furthermore, the cross‑linked immobilized thermophilic esterase exhibited an excellent operational stability, with monomer conversion values exceeding 90% over the course of 12 batch reactions, still more than 80% after 16 batch reactions.
Keywords: immobilized enzyme; cross linking; thermophilic esterase; ring-opening polymerization; ε-caprolactone
PDF Full-text Download PDF Full-Text [330 KB, Updated Version, uploaded 10 July 2014 09:37 CEST]
The original version is still available [320 KB, uploaded 8 July 2014 15:21 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Wang, M.; Shi, H.; Wu, D.; Han, H.; Zhang, J.; Xing, Z.; Wang, S.; Li, Q. Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis. Molecules 2014, 19, 9838-9849.

AMA Style

Wang M, Shi H, Wu D, Han H, Zhang J, Xing Z, Wang S, Li Q. Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis. Molecules. 2014; 19(7):9838-9849.

Chicago/Turabian Style

Wang, Min; Shi, Hui; Wu, Di; Han, Haobo; Zhang, Jianxu; Xing, Zhen; Wang, Shuang; Li, Quanshun. 2014. "Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis." Molecules 19, no. 7: 9838-9849.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert