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Molecules 2014, 19(7), 9838-9849; doi:10.3390/molecules19079838
Article

Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis

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1 Department of Colorectal and Anal Surgery, The Second Hospital, Jilin University, Changchun 130041, China 2 Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China 3 Department of Dermatology, The Second Hospital, Jilin University, Changchun 130041, China
* Authors to whom correspondence should be addressed.
Received: 21 May 2014 / Revised: 3 July 2014 / Accepted: 4 July 2014 / Published: 8 July 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract

The immobilized thermophilic esterase from Archaeoglobus fulgidus was successfully constructed through the glutaraldehyde-mediated covalent coupling after its physical adsorption on a hydrophobic macroporous resin, Sepabeads EC-OD. Through 0.05% glutaraldehyde treatment, the prevention of enzyme leaching and the maintenance of catalytic activity could be simultaneously realized. Using the enzymatic ring-opening polymerization of ε-caprolactone as a model, effects of organic solvents and reaction temperature on the monomer conversion and product molecular weight were systematically investigated. After the optimization of reaction conditions, products were obtained with 100% monomer conversion and Mn values lower than 1010 g/mol. Furthermore, the cross‑linked immobilized thermophilic esterase exhibited an excellent operational stability, with monomer conversion values exceeding 90% over the course of 12 batch reactions, still more than 80% after 16 batch reactions.
Keywords: immobilized enzyme; cross linking; thermophilic esterase; ring-opening polymerization; ε-caprolactone immobilized enzyme; cross linking; thermophilic esterase; ring-opening polymerization; ε-caprolactone
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Wang, M.; Shi, H.; Wu, D.; Han, H.; Zhang, J.; Xing, Z.; Wang, S.; Li, Q. Glutaraldehyde Cross-Linking of Immobilized Thermophilic Esterase on Hydrophobic Macroporous Resin for Application in Poly(ε-caprolactone) Synthesis. Molecules 2014, 19, 9838-9849.

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