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Molecules 2014, 19(7), 9818-9837; doi:10.3390/molecules19079818

Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel

1
Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest H-1111, Hungary
2
SynBiocat LLC., Lázár deák u. 4/1, Budapest H-1173, Hungary
*
Author to whom correspondence should be addressed.
Received: 16 May 2014 / Revised: 23 June 2014 / Accepted: 2 July 2014 / Published: 8 July 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract

Effects of various additives on the lipase from Burkholderia cepacia (BcL) immobilized on mixed-function-grafted mesoporous silica gel support by hydrophobic adsorption and covalent attachment were investigated. Catalytic properties of the immobilized biocatalysts were characterized in kinetic resolution of racemic 1-phenylethanol (rac-1a) and 1-(thiophen-2-yl)ethan-1-ol (rac-1b). Screening of more than 40 additives showed significantly enhanced productivity of immobilized BcL with several additives such as PEGs, oleic acid and polyvinyl alcohol. Effects of substrate concentration and temperature between 0–100 °C on kinetic resolution of rac-1a were studied with the best adsorbed BcLs containing PEG 20 k or PVA 18–88 additives in continuous-flow packed-bed reactor. The optimum temperature of lipase activity for BcL co-immobilized with PEG 20k found at around 30 °C determined in the continuous-flow system increased remarkably to around 80 °C for BcL co-immobilized with PVA 18–88. View Full-Text
Keywords: silica gel; Burkholderia cepacia lipase; enzyme immobilization; adsorption; activity enhancement; additive; continuous-flow packed-bed reactor silica gel; Burkholderia cepacia lipase; enzyme immobilization; adsorption; activity enhancement; additive; continuous-flow packed-bed reactor
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Abaházi, E.; Boros, Z.; Poppe, L. Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel. Molecules 2014, 19, 9818-9837.

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