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Molecules 2014, 19(7), 9818-9837; doi:10.3390/molecules19079818
Article

Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel

1
,
1,2
 and
1,2,*
Received: 16 May 2014 / Revised: 23 June 2014 / Accepted: 2 July 2014 / Published: 8 July 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract

Effects of various additives on the lipase from Burkholderia cepacia (BcL) immobilized on mixed-function-grafted mesoporous silica gel support by hydrophobic adsorption and covalent attachment were investigated. Catalytic properties of the immobilized biocatalysts were characterized in kinetic resolution of racemic 1-phenylethanol (rac-1a) and 1-(thiophen-2-yl)ethan-1-ol (rac-1b). Screening of more than 40 additives showed significantly enhanced productivity of immobilized BcL with several additives such as PEGs, oleic acid and polyvinyl alcohol. Effects of substrate concentration and temperature between 0–100 °C on kinetic resolution of rac-1a were studied with the best adsorbed BcLs containing PEG 20 k or PVA 18–88 additives in continuous-flow packed-bed reactor. The optimum temperature of lipase activity for BcL co-immobilized with PEG 20k found at around 30 °C determined in the continuous-flow system increased remarkably to around 80 °C for BcL co-immobilized with PVA 18–88.
Keywords: silica gel; Burkholderia cepacia lipase; enzyme immobilization; adsorption; activity enhancement; additive; continuous-flow packed-bed reactor silica gel; Burkholderia cepacia lipase; enzyme immobilization; adsorption; activity enhancement; additive; continuous-flow packed-bed reactor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abaházi, E.; Boros, Z.; Poppe, L. Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel. Molecules 2014, 19, 9818-9837.

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