Next Article in Journal
Effect of Structure on the Interactions between Five Natural Antimicrobial Compounds and Phospholipids of Bacterial Cell Membrane on Model Monolayers
Next Article in Special Issue
Antimicrobial Activity of Resveratrol Analogues
Previous Article in Journal
Organic Liquids-Responsive β-Cyclodextrin-Functionalized Graphene-Based Fluorescence Probe: Label-Free Selective Detection of Tetrahydrofuran
Previous Article in Special Issue
Resveratrol and Calcium Signaling: Molecular Mechanisms and Clinical Relevance
Article Menu

Export Article

Open AccessArticle
Molecules 2014, 19(6), 7480-7496; doi:10.3390/molecules19067480

Regulation of Plant Immunity through Modulation of Phytoalexin Synthesis

1
Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA
2
United States Department of Agriculture (USDA), Agricultural Research Service, University of Illinois, 1101 W. Peabody Drive, Urbana, IL 61801, USA
*
Author to whom correspondence should be addressed.
Received: 15 April 2014 / Revised: 29 May 2014 / Accepted: 30 May 2014 / Published: 6 June 2014
(This article belongs to the Special Issue Phytoalexins: Current Progress and Future Prospects)
View Full-Text   |   Download PDF [458 KB, uploaded 18 June 2014]   |  

Abstract

Soybean hairy roots transformed with the resveratrol synthase and resveratrol oxymethyl transferase genes driven by constitutive Arabidopsis actin and CsVMV promoters were characterized. Transformed hairy roots accumulated glycoside conjugates of the stilbenic compound resveratrol and the related compound pterostilbene, which are normally not synthesized by soybean plants. Expression of the non-native stilbenic phytoalexin synthesis in soybean hairy roots increased their resistance to the soybean pathogen Rhizoctonia solani. The expression of the AhRS3 gene resulted in 20% to 50% decreased root necrosis compared to that of untransformed hairy roots. The expression of two genes, the AhRS3 and ROMT, required for pterostilbene synthesis in soybean, resulted in significantly lower root necrosis (ranging from 0% to 7%) in transgenic roots than in untransformed hairy roots that had about 84% necrosis. Overexpression of the soybean prenyltransferase (dimethylallyltransferase) G4DT gene in soybean hairy roots increased accumulation of the native phytoalexin glyceollin resulting in decreased root necrosis. View Full-Text
Keywords: soybean; hairy roots; transformation; phytoalexins; resveratrol; pterostilbene; glyceollins soybean; hairy roots; transformation; phytoalexins; resveratrol; pterostilbene; glyceollins
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zernova, O.V.; Lygin, A.V.; Pawlowski, M.L.; Hill, C.B.; Hartman, G.L.; Widholm, J.M.; Lozovaya, V.V. Regulation of Plant Immunity through Modulation of Phytoalexin Synthesis. Molecules 2014, 19, 7480-7496.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top