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Molecules 2014, 19(6), 7057-7071; doi:10.3390/molecules19067057
Article

Mesoporous Silicas with Tunable Morphology for the Immobilization of Laccase

, ,  and *
Received: 8 May 2014; in revised form: 20 May 2014 / Accepted: 23 May 2014 / Published: 30 May 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract: Siliceous ordered mesoporous materials (OMM) are gaining interest as supports for enzyme immobilization due to their uniform pore size, large surface area, tunable pore network and the introduction of organic components to mesoporous structure. We used SBA-15 type silica materials, which exhibit a regular 2D hexagonal packing of cylindrical mesopores of uniform size, for non-covalent immobilization of laccase. Synthesis conditions were adjusted in order to obtain supports with different particle shape, where those with shorter channels had higher loading capacity. Despite the similar isoelectric points of silica and laccase and the close match between the size of laccase and the pore dimensions of these SBA-15 materials, immobilization was achieved with very low leaching. Surface modification of macro-/mesoporous amorphous silica by grafting of amine moieties was proved to significantly increase the isoelectric point of this support and improve the immobilization yield.
Keywords: amorphous silica; biocatalysts; enzyme immobilization; functionalization; grafting; laccase; MS-3030; ordered mesoporous materials; SBA-15; support amorphous silica; biocatalysts; enzyme immobilization; functionalization; grafting; laccase; MS-3030; ordered mesoporous materials; SBA-15; support
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Gascón, V.; Díaz, I.; Márquez-Álvarez, C.; Blanco, R.M. Mesoporous Silicas with Tunable Morphology for the Immobilization of Laccase. Molecules 2014, 19, 7057-7071.

AMA Style

Gascón V, Díaz I, Márquez-Álvarez C, Blanco RM. Mesoporous Silicas with Tunable Morphology for the Immobilization of Laccase. Molecules. 2014; 19(6):7057-7071.

Chicago/Turabian Style

Gascón, Victoria; Díaz, Isabel; Márquez-Álvarez, Carlos; Blanco, Rosa M. 2014. "Mesoporous Silicas with Tunable Morphology for the Immobilization of Laccase." Molecules 19, no. 6: 7057-7071.



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