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Molecules 2014, 19(6), 7040-7056; doi:10.3390/molecules19067040

Response Surface Optimized Extraction of 1-Deoxynojirimycin from Mulberry Leaves (Morus alba L.) and Preparative Separation with Resins

1
Department of Pharmaceutical Engineering, Beijing Institute of Petro-chemical Technology, Beijing 102617, China
2
Department of Chemical Engineering, Beijing Institute of Petro-chemical Technology, Beijing 102617, China
3
College of Science, Agricultural University of Hebei, Baoding 071001, China
*
Author to whom correspondence should be addressed.
Received: 26 March 2014 / Revised: 21 May 2014 / Accepted: 21 May 2014 / Published: 30 May 2014
(This article belongs to the Section Natural Products)
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Abstract

In the present study, the extraction technology and preparative separation of 1-deoxynojirimycin from mulberry leaves were systematically investigated. Four extraction parameters (ethanol concentration, extraction temperature, extraction time and ratio of solvent to sample) were explored by response surface methodology (RSM). The results indicated that the maximal yield of 1-deoxynojirimycin was achieved with an ethanol concentration of 55%, extraction temperature of 80 °C, extraction time of 1.2 h and ratio of solvent to sample of 12:1. The extraction yield under these optimum conditions was found to be 256 mg/100 g dry mulberry leaves. A column packed with a selected resin was used to perform dynamic adsorption and desorption tests to optimize the separation process. The results show that the preparative separation of 1-deoxynojirimycin from mulberry leaves can be easily and effectively done by adopting 732 resin. In conclusion, 732 resin is the most appropriate for the separation of 1-deoxynojirimycin from other components in mulberry leaves extracts, and its adsorption behavior can be described with Langmuir isotherms and a two-step adsorption kinetics model. The recovery and purity of 1-deoxynojirimycin in the final product were 90.51% and 15.3%, respectively. View Full-Text
Keywords: 1-deoxynojirimycin; extraction; response surface methodology; resin 1-deoxynojirimycin; extraction; response surface methodology; resin
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Wang, T.; Li, C.-Q.; Zhang, H.; Li, J.-W. Response Surface Optimized Extraction of 1-Deoxynojirimycin from Mulberry Leaves (Morus alba L.) and Preparative Separation with Resins. Molecules 2014, 19, 7040-7056.

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