Next Article in Journal
Synthesis, Spectroscopic and Theoretical Studies of New Quaternary N,N-Dimethyl-3-phthalimidopropylammonium Conjugates of Sterols and Bile Acids
Next Article in Special Issue
A Benzoic Acid Derivative and Flavokawains from Piper species as Schistosomiasis Vector Controls
Previous Article in Journal
Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa
Previous Article in Special Issue
Antischistosomal Activity of the Terpene Nerolidol
Molecules 2014, 19(4), 4200-4211; doi:10.3390/molecules19044200

Antiprotozoal Activities of Millettia richardiana (Fabaceae) from Madagascar

, 2,4,* , 5,6,7
, 4
, 8
, 8
, 2
, 1
, 3
 and 5,6,7,*
Received: 21 January 2014 / Revised: 18 March 2014 / Accepted: 19 March 2014 / Published: 3 April 2014
View Full-Text   |   Download PDF [224 KB, uploaded 18 June 2014]
Abstract: With at least 60% of the Millettia species (Fabaceae) being in medicinal use, we found it relevant to assess the potential antiprotozoal and antifungal activities of Millettia richardiana. Water and methanol crude extracts of the stem barks from M. richardiana and the six fractions resulting from the fractionation of the methanol extract were tested. The dichloromethane extracted fraction showed the best in vitro antiprotozoal activities (IC50 = 5.8 μg/mL against Plasmodium falciparum, 11.8 μg/mL against Leishmania donovani and 12.8 μg/mL against Trypanosoma brucei brucei) as well as low cytotoxicity on several cell lines. The phytochemical analysis showed this selected fraction to be rich in terpenoids and alkaloids, which could explain its antiparasitic activity. A phytochemical study revealed the presence of lonchocarpenin, betulinic acid, β-amyrin, lupeol, palmitic acid, linoleic acid and stearic acid, among which betulinic acid and lupeol could be the compounds responsible of these antiprotozoal activities. By contrast, neither the crude extracts nor the fractions showed antifungal activity against Candida. These results confirm the importance of the genus Millettia in Malagasy ethnomedicine, its potential use in antiparasitic therapy, and the interest of developing a sustainable exploitation of this plant. Moreover, both molecules betulinic acid and lupeol appeared as very relevant molecules for their antiprotozoal properties.
Keywords: Millettia richardiana; traditional medicine; Madagascar; Plasmodium; Trypanosoma; Leishmania; Candida Millettia richardiana; traditional medicine; Madagascar; Plasmodium; Trypanosoma; Leishmania; Candida
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |

MDPI and ACS Style

Rajemiarimiraho, M.; Banzouzi, J.-T.; Nicolau-Travers, M.-L.; Ramos, S.; Cheikh-Ali, Z.; Bories, C.; Rakotonandrasana, O.L.; Rakotonandrasana, S.; Andrianary, P.A.; Benoit-Vical, F. Antiprotozoal Activities of Millettia richardiana (Fabaceae) from Madagascar. Molecules 2014, 19, 4200-4211.

AMA Style

Rajemiarimiraho M, Banzouzi J-T, Nicolau-Travers M-L, Ramos S, Cheikh-Ali Z, Bories C, Rakotonandrasana OL, Rakotonandrasana S, Andrianary PA, Benoit-Vical F. Antiprotozoal Activities of Millettia richardiana (Fabaceae) from Madagascar. Molecules. 2014; 19(4):4200-4211.

Chicago/Turabian Style

Rajemiarimiraho, Manitriniaina; Banzouzi, Jean-Théophile; Nicolau-Travers, Marie-Laure; Ramos, Suzanne; Cheikh-Ali, Zakaria; Bories, Christian; Rakotonandrasana, Olga L.; Rakotonandrasana, Stéphane; Andrianary, Philippe A.; Benoit-Vical, Françoise. 2014. "Antiprotozoal Activities of Millettia richardiana (Fabaceae) from Madagascar." Molecules 19, no. 4: 4200-4211.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert