Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa
Abstract
:1. Introduction
2. Results and Discussion
Concentration | 0.1 mg/mL * | 0.05 mg/mL | 0.025 mg/mL |
---|---|---|---|
A. blazei extract | 16.06 ± 0.47 | 42.58 ± 0.35 | 98.37 ± 0.97 |
Ampicillin | 69.16 ± 0.65 | 56.46 ± 0.46 | 92.16 ± 0.37 |
Streptomycin | 49.40 ± 0.46 | 70.97 ± 0.36 | 88.36 ± 0.42 |
P. aeruginosa control | 98.90 ± 0.97 | 100.00 ± 0.00 | 99.30 ± 0.46 |
Concentration mg/disc | 0.03 | 0.06 | 0.125 | 0.25 | 0.50 | |
---|---|---|---|---|---|---|
A. blazei extr. | MIC | - | - | - | - | - |
AQ | 7.0 ± 1.0 | 7.3 ± 0.5 | 8.0 ± 1.0 | 9.3 ± 1.0 | 17.7 ± 1.0 | |
Ampicillin | MIC | - | - | - | - | - |
AQ | -- | -- | -- | 7.6 ± 0.6 | 9.0 ± 1.0 | |
Streptomycin | MIC | - | - | 9.0 ± 1.0 15.0 ± 2.1 | 16.6 ± 1.5 22.6 ± 2.3 | 18.6 ± 1.2 26.7 ± 1.2 |
AQ | ||||||
P. aeruginosa Control | MIC AQ | ni | ni | ni | ni | ni |
Extracts | Colony diameter | Colony color | Colony edge |
---|---|---|---|
A. blazei H2O | 21.67 ± 0.51 | white | reduced flagella |
Streptomycin | 5.00 ± 0.06 | white | flat |
Ampicillin | 12.00 ± 1.00 | white | regular |
Control P. aeruginosa 109 CFU | 14.00 ± 1.00 | green | regular |
3. Experimental
3.1. Extract Preparation
3.2. Bacterial Strains, Growth Media and Culture Conditions
3.3. Biofilm Formation
3.4. Inhibition of Biofilm Formation of P. aeruginosa
3.5. Inhibition of Twitching and Flagella Motility of P. aeruginosa
3.6. Inhibition of Synthesis of P. aeruginosa PA01 Pyocyanin
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Soković, M.; Ćirić, A.; Glamočlija, J.; Nikolić, M.; Van Griensven, L.J.L.D. Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa. Molecules 2014, 19, 4189-4199. https://doi.org/10.3390/molecules19044189
Soković M, Ćirić A, Glamočlija J, Nikolić M, Van Griensven LJLD. Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa. Molecules. 2014; 19(4):4189-4199. https://doi.org/10.3390/molecules19044189
Chicago/Turabian StyleSoković, Marina, Ana Ćirić, Jasmina Glamočlija, Miloš Nikolić, and Leo J. L. D. Van Griensven. 2014. "Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas Aeruginosa" Molecules 19, no. 4: 4189-4199. https://doi.org/10.3390/molecules19044189