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Recent Advances in Target Characterization and Identification by Photoaffinity Probes
BioNanotechnology Research Center, KRIBB, 125 Kwahangno, Yuseong, Daejeon 305-806, Korea
National Nanotechnology Center (NANOTEC), 111 Phahonyothin Rd., Klongluang, Pathumthani 12120, Thailand
Department of Chemistry, Dongguk University, 26 Phil-dong, Jung-gu, Seoul 100-715, Korea
* Author to whom correspondence should be addressed.
Received: 30 July 2013; in revised form: 23 August 2013 / Accepted: 23 August 2013 / Published: 29 August 2013
Abstract: Target identification of biologically active molecules such as natural products, synthetic small molecules, peptides, and oligonucleotides mainly relies on affinity chromatography, activity-based probes, or photoaffinity labeling (PAL). Amongst them, activity-based probes and PAL have offered great advantages in target identification technology due to their ability to form covalent bonds with the corresponding targets. Activity-based probe technology mainly relies on the chemical reactivity of the target proteins, thereby limiting the majority of the biological targets to enzymes or proteins which display reactive residues at the probe-binding site. In general, the probes should bear a reactive moiety such as an epoxide, a Michael acceptor, or a reactive alkyl halide in their structures. On the other hand, photoaffinity probes (PAPs) are composed of a target-specific ligand and a photoactivatable functional group. When bound to the corresponding target proteins and activated with wavelength-specific light, PAPs generate highly reactive chemical species that covalently cross-link proximal amino acid residues. This process is better known as PAL and is widely employed to identify cellular targets of biologically active molecules. This review highlights recent advances in target identification by PAL, with a focus on the structure and chemistry of the photoaffinity probes developed in the recent decade, coupled to the target proteins identified using these probes.
Keywords: protein target identification; photoaffinity labeling; affinity chromatography; chemical proteomics; photo-crosslinking; bioorthogonal ligation; two step labeling; click chemistry
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MDPI and ACS Style
Sumranjit, J.; Chung, S.J. Recent Advances in Target Characterization and Identification by Photoaffinity Probes. Molecules 2013, 18, 10425-10451.
Sumranjit J, Chung SJ. Recent Advances in Target Characterization and Identification by Photoaffinity Probes. Molecules. 2013; 18(9):10425-10451.
Sumranjit, Jitapa; Chung, Sang J. 2013. "Recent Advances in Target Characterization and Identification by Photoaffinity Probes." Molecules 18, no. 9: 10425-10451.