Molecules 2013, 18(9), 11639-11657; doi:10.3390/molecules180911639
Article

A Modular Approach to Triazole-Containing Chemical Inducers of Dimerisation for Yeast Three-Hybrid Screening

Received: 1 August 2013; in revised form: 5 September 2013 / Accepted: 6 September 2013 / Published: 23 September 2013
(This article belongs to the Special Issue Reagents and Methods for Protein Target Identification)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The yeast three-hybrid (Y3H) approach shows considerable promise for the unbiased identification of novel small molecule-protein interactions. In recent years, it has been successfully used to link a number of bioactive molecules to novel protein binding partners. However despite its potential importance as a protein target identification method, the Y3H technique has not yet been widely adopted, in part due to the challenges associated with the synthesis of the complex chemical inducers of dimerisation (CIDs). The development of a modular approach using potentially “off the shelf” synthetic components was achieved and allowed the synthesis of a family of four triazole-containing CIDs, MTX-Cmpd2.2-2.5. These CIDs were then compared using the Y3H approach with three of them giving a strong positive interaction with a known target of compound 2, TgCDPK1. These results showed that the modular nature of our synthetic strategy may help to overcome the challenges currently encountered with CID synthesis and should contribute to the Y3H approach reaching its full potential as an unbiased target identification strategy.
Keywords: click chemistry; yeast three-hybrid approach; CIDs
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MDPI and ACS Style

Tran, F.; Odell, A.V.; Ward, G.E.; Westwood, N.J. A Modular Approach to Triazole-Containing Chemical Inducers of Dimerisation for Yeast Three-Hybrid Screening. Molecules 2013, 18, 11639-11657.

AMA Style

Tran F, Odell AV, Ward GE, Westwood NJ. A Modular Approach to Triazole-Containing Chemical Inducers of Dimerisation for Yeast Three-Hybrid Screening. Molecules. 2013; 18(9):11639-11657.

Chicago/Turabian Style

Tran, Fanny; Odell, Anahi V.; Ward, Gary E.; Westwood, Nicholas J. 2013. "A Modular Approach to Triazole-Containing Chemical Inducers of Dimerisation for Yeast Three-Hybrid Screening." Molecules 18, no. 9: 11639-11657.

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