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Molecules 2013, 18(8), 9999-10013; doi:10.3390/molecules18089999
Article

Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery

1,2,†, 3,†, 3, 3, 3, 2, 4, 2, 2, 3 and 1,2,4,*
Received: 26 June 2013 / Revised: 13 August 2013 / Accepted: 14 August 2013 / Published: 20 August 2013
(This article belongs to the Special Issue Reagents and Methods for Protein Target Identification)
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Abstract

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1–10 micromolar range, they were non-toxic even during long-term incubations.
Keywords: fluorescent dye discovery; high-throughput screening; small molecule microarray; confocal laser scanning microscopy fluorescent dye discovery; high-throughput screening; small molecule microarray; confocal laser scanning microscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Molnár, E.; Kuntam, S.; Cingaram, P.K.R.; Peksel, B.; Suresh, B.; Fábián, G.; Fehér, L.Z.; Bokros, A.; Medgyesi, Á.; Ayaydin, F.; Puskás, L.G. Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery. Molecules 2013, 18, 9999-10013.

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