Molecules 2013, 18(7), 8393-8401; doi:10.3390/molecules18078393
Article

Distinct Metabolites for Photoreactive l-Phenylalanine Derivatives in Klebsiella sp. CK6 Isolated from Rhizosphere of a Wild Dipterocarp Sapling

1 Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University; Kita 9, Nishi 9, Kita-ku, Sapporo 060-8589, Japan 2 Forest Microbiology Laboratory, Forest and Nature Conservation Research and Development Center, Forest Research and Development Agency, Jalan Gunung Batu No.5, P.O. Box 165, Bogor 16610, Indonesia 3 Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan
* Author to whom correspondence should be addressed.
Received: 14 June 2013; in revised form: 2 July 2013 / Accepted: 11 July 2013 / Published: 16 July 2013
(This article belongs to the Special Issue Reagents and Methods for Protein Target Identification)
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Abstract: Photoaffinity labeling is a reliable analytical method for biological functional analysis. Three major photophores—aryl azide, benzophenone and trifluoromethyldiazirine—are utilized in analysis. Photophore-bearing l-phenylalanine derivatives, which are used for biological functional analysis, were inoculated into a Klebsiella sp. isolated from the rhizosphere of a wild dipterocarp sapling in Central Kalimantan, Indonesia, under nitrogen-limiting conditions. The proportions of metabolites were quite distinct for each photophore. These results indicated that photophores affected substrate recognition in rhizobacterial metabolic pathways, and differential photoaffinity labeling could be achieved using different photophore-containing l-phenylalanine derivatives.
Keywords: photoaffinity label; phenylalanine; tryptophan; phenyl azide; benzophenone; trifluoromethyldiazirine; metabolites

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MDPI and ACS Style

Wang, L.; Hisano, W.; Murai, Y.; Sakurai, M.; Muto, Y.; Ikemoto, H.; Okamoto, M.; Murotani, T.; Isoda, R.; Kim, D.; Sakihama, Y.; Sitepu, I.R.; Hashidoko, Y.; Hatanaka, Y.; Hashimoto, M. Distinct Metabolites for Photoreactive l-Phenylalanine Derivatives in Klebsiella sp. CK6 Isolated from Rhizosphere of a Wild Dipterocarp Sapling. Molecules 2013, 18, 8393-8401.

AMA Style

Wang L, Hisano W, Murai Y, Sakurai M, Muto Y, Ikemoto H, Okamoto M, Murotani T, Isoda R, Kim D, Sakihama Y, Sitepu IR, Hashidoko Y, Hatanaka Y, Hashimoto M. Distinct Metabolites for Photoreactive l-Phenylalanine Derivatives in Klebsiella sp. CK6 Isolated from Rhizosphere of a Wild Dipterocarp Sapling. Molecules. 2013; 18(7):8393-8401.

Chicago/Turabian Style

Wang, Lei; Hisano, Wataru; Murai, Yuta; Sakurai, Munenori; Muto, Yasuyuki; Ikemoto, Haruka; Okamoto, Masashi; Murotani, Takashi; Isoda, Reika; Kim, Dongyeop; Sakihama, Yasuko; Sitepu, Irnayuli R.; Hashidoko, Yasuyuki; Hatanaka, Yasumaru; Hashimoto, Makoto. 2013. "Distinct Metabolites for Photoreactive l-Phenylalanine Derivatives in Klebsiella sp. CK6 Isolated from Rhizosphere of a Wild Dipterocarp Sapling." Molecules 18, no. 7: 8393-8401.

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