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Molecules 2013, 18(7), 8402-8416; doi:10.3390/molecules18078402

Characterization of Flavonoids and Phenolic Acids in Myrcia bella Cambess. Using FIA-ESI-IT-MSn and HPLC-PAD-ESI-IT-MS Combined with NMR

1, 2 and 3,*
1 Botany Department, Institute of Biosciences, Univ. Estadual Paulista (UNESP), CEP 18618-970, Botucatu, Sao Paulo, Brazil 2 Experimental Campus of the Paulista Coast, Univ. Estadual Paulista (UNESP), CEP 11330-900, Sao Vicente, Sao Paulo, Brazil 3 Biological Science Department, Science Faculty, Univ. Estadual Paulista (UNESP), CEP 17033-360, Bauru, Sao Paulo, Brazil
* Author to whom correspondence should be addressed.
Received: 7 June 2013 / Revised: 5 July 2013 / Accepted: 8 July 2013 / Published: 16 July 2013
(This article belongs to the Section Metabolites)
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The leaves of Myrcia DC. ex Guill species are used in traditional medicine and are also exploited commercially as herbal drugs for the treatment of diabetes mellitus. The present work aimed to assess the qualitative and quantitative profiles of M. bella hydroalcoholic extract, due to these uses, since the existing legislation in Brazil determines that a standard method must be developed in order to be used for quality control of raw plant materials. The current study identified eleven known flavonoid-O-glycosides and six acylated flavonoid derivatives of myricetin and quercetin, together with two kaempferol glycosides and phenolic acids such as caffeic acid, ethil galate, gallic acid and quinic acid. In total, 24 constituents were characterized, by means of extensive preparative chromatographic analyses, along with MS and NMR techniques. An HPLC-PAD-ESI-IT-MS and FIA-ESI-IT-MSn method were developed for rapid identification of acylated flavonoids, flavonoid-O-glycosides derivatives of myricetin and quercetin and phenolic acids in the hydroalcoholic M. bella leaves extract. The FIA-ESI-IT-MS techinique is a powerful tool for direct and rapid identification of the constituents after isolation and NMR characterization. Thus, it could be used as an initial method for identification of authentic samples concerning quality control of Myrcia spp extracts.
Keywords: Brazilian savanna; flavonoid-O-glycosides; medicinal plants; Myrtaceae Brazilian savanna; flavonoid-O-glycosides; medicinal plants; Myrtaceae
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Saldanha, L.L.; Vilegas, W.; Dokkedal, A.L. Characterization of Flavonoids and Phenolic Acids in Myrcia bella Cambess. Using FIA-ESI-IT-MSn and HPLC-PAD-ESI-IT-MS Combined with NMR. Molecules 2013, 18, 8402-8416.

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