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Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability
Institute of Cardiovascular Disease Research, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China
Department of Cardiology, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China
* Author to whom correspondence should be addressed.
Received: 2 May 2013; in revised form: 3 July 2013 / Accepted: 4 July 2013 / Published: 9 July 2013
Abstract: Luteolin (Lut) is a common dietary flavonoid present in Chinese herbal medicines that has been reported to have important anti-inflammatory properties. The purposes of this study were to observe the inhibition of lipopolysaccharide (LPS)-induced inflammatory responses in bone marrow macrophages (BMM) by Lut, and to examine whether this inhibition involves p38/MK2/TTP-mediated mRNA stability. Lut suppressed the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in a dose-dependent manner according to enzyme-linked immunosorbent assay (ELISA) analysis. Lut also shortened the half-lives of the TNF-α and IL-6 mRNAs according to real-time PCR analysis. Western blots were performed to assess the activation of p38 and MK2 as well as the expression of TTP. The results indicated that Lut inhibited p38 and MK2 phosphorylation while promoting TTP expression. These results suggest that the anti-inflammatory effects of Lut are partially mediated through p38/MK2/TTP-regulated mRNA stability.
Keywords: luteolin; anti-inflammatory; bone marrow macrophages; mRNA stability
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MDPI and ACS Style
Wu, W.; Li, D.; Zong, Y.; Zhu, H.; Pan, D.; Xu, T.; Wang, T.; Wang, T. Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability. Molecules 2013, 18, 8083-8094.
Wu W, Li D, Zong Y, Zhu H, Pan D, Xu T, Wang T, Wang T. Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability. Molecules. 2013; 18(7):8083-8094.
Wu, Wanling; Li, Dongye; Zong, Yu; Zhu, Hong; Pan, Defeng; Xu, Tongda; Wang, Tao; Wang, Tingting. 2013. "Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability." Molecules 18, no. 7: 8083-8094.