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Molecules 2013, 18(7), 8083-8094; doi:10.3390/molecules18078083

Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability

1, 1,* , 1, 2, 2, 2, 1 and 1
1 Institute of Cardiovascular Disease Research, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou 221002, Jiangsu, China 2 Department of Cardiology, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou 221002, Jiangsu, China
* Author to whom correspondence should be addressed.
Received: 2 May 2013 / Revised: 3 July 2013 / Accepted: 4 July 2013 / Published: 9 July 2013
(This article belongs to the Special Issue Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry)
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Luteolin (Lut) is a common dietary flavonoid present in Chinese herbal medicines that has been reported to have important anti-inflammatory properties. The purposes of this study were to observe the inhibition of lipopolysaccharide (LPS)-induced inflammatory responses in bone marrow macrophages (BMM) by Lut, and to examine whether this inhibition involves p38/MK2/TTP-mediated mRNA stability. Lut suppressed the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in a dose-dependent manner according to enzyme-linked immunosorbent assay (ELISA) analysis. Lut also shortened the half-lives of the TNF-α and IL-6 mRNAs according to real-time PCR analysis. Western blots were performed to assess the activation of p38 and MK2 as well as the expression of TTP. The results indicated that Lut inhibited p38 and MK2 phosphorylation while promoting TTP expression. These results suggest that the anti-inflammatory effects of Lut are partially mediated through p38/MK2/TTP-regulated mRNA stability.
Keywords: luteolin; anti-inflammatory; bone marrow macrophages; mRNA stability luteolin; anti-inflammatory; bone marrow macrophages; mRNA stability
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Wu, W.; Li, D.; Zong, Y.; Zhu, H.; Pan, D.; Xu, T.; Wang, T.; Wang, T. Luteolin Inhibits Inflammatory Responses via p38/MK2/TTP-mediated mRNA Stability. Molecules 2013, 18, 8083-8094.

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