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Molecules 2013, 18(7), 8095-8108; doi:10.3390/molecules18078095
Article

Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study

1, 1,†
, 2
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 and 1,*
Received: 27 May 2013; in revised form: 5 July 2013 / Accepted: 5 July 2013 / Published: 9 July 2013
(This article belongs to the Section Metabolites)
Download PDF [328 KB, uploaded 18 June 2014]
Abstract: In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level.
Keywords: Plinia cauliflora (Myrtaceae); ellagitannin; Candida; antifungal activity; cell wall Plinia cauliflora (Myrtaceae); ellagitannin; Candida; antifungal activity; cell wall
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Souza-Moreira, T.M.; Severi, J.A.; Lee, K.; Preechasuth, K.; Santos, E.; Gow, N.A.R.; Munro, C.A.; Vilegas, W.; Pietro, R.C.L.R. Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study. Molecules 2013, 18, 8095-8108.

AMA Style

Souza-Moreira TM, Severi JA, Lee K, Preechasuth K, Santos E, Gow NAR, Munro CA, Vilegas W, Pietro RCLR. Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study. Molecules. 2013; 18(7):8095-8108.

Chicago/Turabian Style

Souza-Moreira, Tatiana M.; Severi, Juliana A.; Lee, Keunsook; Preechasuth, Kanya; Santos, Emerson; Gow, Neil A.R.; Munro, Carol A.; Vilegas, Wagner; Pietro, Rosemeire C.L.R. 2013. "Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study." Molecules 18, no. 7: 8095-8108.


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