In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria
Abstract
:1. Introduction
2. Results and Discussion
Bacteria | MIC in µg mL−1 (MIC in µM) | |||
---|---|---|---|---|
Positive control 1 | MO | KA | CA 2 | |
S. aureus (ATCC 6538) | 0.05 (0.05) | * | * | 6.25 (20.54) |
S. aureus (clinical isolate) | >5.89 (>6.48) | 200.00 (689.03) | * | 6.25 (20.54) |
E. coli (ATCC 14948) | >1.50 (>1.65) | * | * | * |
E. coli (clinical isolate) | >2.95 (>3.25) | * | * | * |
S. epidermidis (ATCC 12228) | 0.05 (0.05) | 100.00 (344.51) | 25.00 (82.78) | 6.25 (20.54) |
S. agalactiae (ATCC 12386) | 0.05 (0.05) | 6.25 (21.53) | 3.12 (10.33) | 1.56 (5.13) |
S. dysgalactiae (ATCC 12238) | 0.05 (0.05) | 6.25 (21.53) | 3.12 (10.33) | 1.56 (5.13) |
3. Experimental
3.1. Compound Isolation and Identification
3.2. Bacterial Strains and Antimicrobial Testing
3.3. Kill Kinetics
3.4. Cytotoxicity Assay
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Fonseca, A.P.; Estrela, F.T.; Moraes, T.S.; Carneiro, L.J.; Bastos, J.K.; Santos, R.A.d.; Ambrósio, S.R.; Martins, C.H.G.; Veneziani, R.C.S. In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria. Molecules 2013, 18, 7865-7872. https://doi.org/10.3390/molecules18077865
Fonseca AP, Estrela FT, Moraes TS, Carneiro LJ, Bastos JK, Santos RAd, Ambrósio SR, Martins CHG, Veneziani RCS. In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria. Molecules. 2013; 18(7):7865-7872. https://doi.org/10.3390/molecules18077865
Chicago/Turabian StyleFonseca, Ariana P., Fernanda T. Estrela, Thaís S. Moraes, Luiza J. Carneiro, Jairo K. Bastos, Raquel A. dos Santos, Sérgio R. Ambrósio, Carlos H. G. Martins, and Rodrigo C. S. Veneziani. 2013. "In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria" Molecules 18, no. 7: 7865-7872. https://doi.org/10.3390/molecules18077865