Next Article in Journal
Comprehensive Theoretical Studies on the Reaction of 1-Bromo-3,3,3-trifluoropropene with OH Free Radicals
Previous Article in Journal
Characterization of Protein and Peptide Binding to Nanogels Formed by Differently Charged Chitosan Derivatives
Molecules 2013, 18(7), 7865-7872; doi:10.3390/molecules18077865

In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria

1, 1, 1, 1, 2, 1, 1, 1,*  and 1,*
1 Nucleus of Research in Sciences and Technology, University of Franca, Armando Salles de Oliveira Road, 201, Franca 1404-600, Brazil 2 Faculty of Pharmaceutical Sciences of Ribeirão Preto, Laboratory of Pharmacognosy, University of São Paulo, Café Avenue, Ribeirão Preto 14040-903, Brazil
* Authors to whom correspondence should be addressed.
Received: 27 May 2013 / Revised: 20 June 2013 / Accepted: 21 June 2013 / Published: 4 July 2013
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [214 KB, 18 June 2014; original version 18 June 2014]   |   Browse Figures


We evaluated the antibacterial activity of three diterpenes isolated from natural sources against a panel of microorganisms responsible for bovine mastitis. ent-Copalic acid (CA) was the most active metabolite, with promising MIC values (from 1.56 to 6.25 µg mL−1) against Staphylococcus aureus (ATCC and clinical isolate), Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus dysgalactiae. We conducted time-kill assays of CA against S. aureus, a commensal organism considered to be a ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. In the first 12 h, CA only inhibited the growth of the inoculums (bacteriostatic effect), but its bactericidal effect was clearly noted thereafter (between 12 and 24 h). In conclusion, CA should be considered for the control of several Gram-positive bacteria related to bovine mastitis.
Keywords: diterpenes; bovine mastitis; antibacterial; ent-copalic acid diterpenes; bovine mastitis; antibacterial; ent-copalic acid
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Fonseca, A.P.; Estrela, F.T.; Moraes, T.S.; Carneiro, L.J.; Bastos, J.K.; Santos, R.A.; Ambrósio, S.R.; Martins, C.H.G.; Veneziani, R.C.S. In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria. Molecules 2013, 18, 7865-7872.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert