Abstract: Sophoricoside exhibits numerous pharmacological effects, including anti- inflammatory and anti-cancer actions, yet the exact mechanism that accounts for the anti-allergic effects of sophoricoside is not completely understood. The aim of the present study was to elucidate whether and how sophoricoside modulates the mast cell-mediated allergic inflammation in vitro and in vivo. We investigated the pharmacological effects of sophoricoside on both compound 48/80 or histamine-induced scratching behaviors and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of sophoricoside, we evaluated the effects of sophoricoside on the production of histamine and inflammatory cytokines and activation of nuclear factor-κB (NF-κB) and caspase-1 in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1). The finding of this study demonstrated that sophoricoside reduced compound 48/80 or histamine-induced scratching behaviors and DNCB-induced atopic dermatitis in mice. Additionally, sophoricoside inhibited the production of inflammatory cytokines as well as the activation of NF-κB and caspase-1 in stimulated HMC-1. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of sophoricoside as a potential molecule for use in the treatment of allergic inflammation diseases.
Keywords: sophoricoside; inflammatory cytokine; mast cells; allergic inflammation
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Kim, S.-J.; Lee, G.-Y.; Jung, J.-W.; Oh, S.-R.; Ahn, E.-M.; Kim, S.-H.; Hong, S.-H.; Um, J.-Y. The Ameliorative Effect of Sophoricoside on Mast Cell-Mediated Allergic Inflammation in Vivo and in Vitro. Molecules 2013, 18, 6113-6127.
Kim S-J, Lee G-Y, Jung J-W, Oh S-R, Ahn E-M, Kim S-H, Hong S-H, Um J-Y. The Ameliorative Effect of Sophoricoside on Mast Cell-Mediated Allergic Inflammation in Vivo and in Vitro. Molecules. 2013; 18(5):6113-6127.
Kim, Su-Jin; Lee, Gil-Yong; Jung, Ji-Wook; Oh, Sa-Rang; Ahn, Eun-Mi; Kim, Sung-Hoon; Hong, Seung-Heon; Um, Jae-Young. 2013. "The Ameliorative Effect of Sophoricoside on Mast Cell-Mediated Allergic Inflammation in Vivo and in Vitro." Molecules 18, no. 5: 6113-6127.