Next Article in Journal
Antioxidant Activity and Proanthocyanidin Profile of Selliguea feei Rhizomes
Next Article in Special Issue
Preparation, Purification and Regioselective Functionalization of Protoescigenin—The Main Aglycone of Escin Complex
Previous Article in Journal
Two New Monoterpene Glycosides from Qing Shan Lu Shui Tea with Inhibitory Effects on Leukocyte-Type 12-Lipoxygenase Activity
Previous Article in Special Issue
Triterpene Esters: Natural Products from Dorstenia arifolia (Moraceae)
Article Menu

Export Article

Open AccessArticle
Molecules 2013, 18(4), 4267-4281; doi:10.3390/molecules18044267

Metabolomic Analysis of Methyl Jasmonate-Induced Triterpenoid Production in the Medicinal Herb Centella asiatica (L.) Urban

Department of Biochemistry, University of Johannesburg, Auckland Park 2006, South Africa
Drug Discovery Division, BioSciences, CSIR, Pretoria 0001, South Africa
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 31 January 2013 / Revised: 3 April 2013 / Accepted: 3 April 2013 / Published: 11 April 2013
(This article belongs to the Special Issue Triterpenes and Triterpenoids)
View Full-Text   |   Download PDF [245 KB, uploaded 18 June 2014]   |  


Centella asiatica is an important source of biologically active pentacyclic triterpenoids. The enhancement of the biosynthesis of the centellosides by manipulation of associated metabolic pathways is receiving much attention. Jasmonates play critical roles in plant metabolism by up-regulating the expression of genes related to secondary metabolites. Here, we investigated the effect of methyl jasmonate (MeJa) in C. asiatica through targeted metabolomic profiling of asiaticoside and madecassoside as well as their aglycones, asiatic acid and madecassic acid. Cell suspensions were treated with 0.2 mM MeJa for 2, 4 and 6 days. Liquid chromatography coupled to mass spectrometry (LC-MS) was used to explore induced changes in metabolite profiles, both qualitatively and quantitatively. Principal component analysis (PCA)-derived scores plots revealed clusters of sample replicates for control and treated samples at 2, 4 and 6 days while loading plots aided in identifying signatory biomarkers (asiatic acid and madecassic acid, as well as asiaticoside and madecassoside) that clearly demonstrate the variability between samples. In addition to increased biosynthesis of the targeted centelloids, other differential changes in the intracellular metabolite profiles reflected the response of the C. asiatica cells to the MeJa-treatment as a reprogramming of the metabolome. View Full-Text
Keywords: Centella asiatica; centelloids; triterpenoids; metabolic profiling; metabolomics; LC-MS Centella asiatica; centelloids; triterpenoids; metabolic profiling; metabolomics; LC-MS

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

James, J.T.; Tugizimana, F.; Steenkamp, P.A.; Dubery, I.A. Metabolomic Analysis of Methyl Jasmonate-Induced Triterpenoid Production in the Medicinal Herb Centella asiatica (L.) Urban. Molecules 2013, 18, 4267-4281.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top