Molecules 2012, 17(8), 9207-9219; doi:10.3390/molecules17089207
Article

Real Time Anti-Toxoplasma gondii Activity of an Active Fraction of Eurycoma longifolia Root Studied by in Situ Scanning and Transmission Electron Microscopy

1, 1, 2 and 1,* email
Received: 25 June 2012; in revised form: 8 July 2012 / Accepted: 10 July 2012 / Published: 2 August 2012
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The inhibitory effect of active fractions of Eurycoma longifolia (E. longifolia) root, namely TAF355 and TAF401, were evaluated against Toxoplasma gondii (T. gondii). In our previous study, we demonstrated that T. gondii was susceptible to TAF355 and TAF401 with IC50 values of 1.125 µg/mL and 1.375 µg/mL, respectively. Transmission (TEM) and scanning electron microscopy (SEM) observations were used to study the in situ antiparasitic activity at the IC50 value. Clindamycin was used as positive control. SEM examination revealed cell wall alterations with formation of invaginations followed by completely collapsed cells compared to the normal T. gondii cells in response to the fractions. The main abnormality noted via TEM study was decreased cytoplasmic volume, leaving a state of structural disorganization within the cell cytoplasm and destruction of its organelles as early as 12 h of treatment, which indicated of rapid antiparasitic activity of the E. longifolia fractions. The significant antiparasitic activity shown by the TAF355 and TAF401 active fractions of E. longifolia suggests their potential as new anti-T. gondii agent candidates.
Keywords: Toxoplasma gondii; Eurycoma longifolia; transmission electron microscope; scanning electron microscope; toxoplasmosis
PDF Full-text Download PDF Full-Text [3370 KB, uploaded 18 June 2014 19:51 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Kavitha, N.; Noordin, R.; Kit-Lam, C.; Sasidharan, S. Real Time Anti-Toxoplasma gondii Activity of an Active Fraction of Eurycoma longifolia Root Studied by in Situ Scanning and Transmission Electron Microscopy. Molecules 2012, 17, 9207-9219.

AMA Style

Kavitha N, Noordin R, Kit-Lam C, Sasidharan S. Real Time Anti-Toxoplasma gondii Activity of an Active Fraction of Eurycoma longifolia Root Studied by in Situ Scanning and Transmission Electron Microscopy. Molecules. 2012; 17(8):9207-9219.

Chicago/Turabian Style

Kavitha, Nowroji; Noordin, Rahmah; Kit-Lam, Chan; Sasidharan, Sreenivasan. 2012. "Real Time Anti-Toxoplasma gondii Activity of an Active Fraction of Eurycoma longifolia Root Studied by in Situ Scanning and Transmission Electron Microscopy." Molecules 17, no. 8: 9207-9219.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert