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Molecules 2012, 17(8), 8859-8871; doi:10.3390/molecules17088859
Communication

Amperometric Biosensor for Oxalate Determination in Urine Using Sequential Injection Analysis

1,* , 1
, 1
, 2
 and 2
1 Chemical Research Center, Universidad Autonoma el Estado de Hidalgo, Carr. Pachuca-Tulancingo km 4.5, 42076, Pachuca, Hidalgo, Mexico 2 Analytical Chemistry Department, Faculty of Sciences, Universidad de Valladolid, Campus Miguel Delibes s/n, 47011 Valladolid, Spain
* Author to whom correspondence should be addressed.
Received: 16 May 2012 / Revised: 9 July 2012 / Accepted: 11 July 2012 / Published: 26 July 2012
(This article belongs to the Special Issue Flow Chemistry)
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Abstract

An amperometric flow biosensor for oxalate determination in urine samples after enzymatic reaction with oxalate oxidase immobilized on a modified magnetic solid is described. The solid was magnetically retained on the electrode surface of an electrode modified with Fe (III)-tris-(2-thiopyridone) borate placed into a sequential injection system preceding the amperometric detector. The variables involved in the system such as flow rate, aspired volumes (modified magnetic suspension and sample) and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of oxalate was linear between 3.0–50.0 mg·L−1, with a limit of detection of 1.0 mg·L−1. The repeatability for a 30.0 mg·L−1 oxalate solution was 0.7%. The method was validated by comparing the obtained results to those provided by the spectrophotometric method; no significant differences were observed.
Keywords: oxalate; urine; sequential injection analysis; magnetic particles oxalate; urine; sequential injection analysis; magnetic particles
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Rodriguez, J.A.; Hernandez, P.; Salazar, V.; Castrillejo, Y.; Barrado, E. Amperometric Biosensor for Oxalate Determination in Urine Using Sequential Injection Analysis. Molecules 2012, 17, 8859-8871.

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