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Antiatherogenic Properties of Acetone Extract of Alpinia zerumbet Seeds
Department of Bioscience and Biotechnology, The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima 890-0065, Japan
Instrumental Research Center, University of the Ryukyus, Senbaru 1, Nishihara-cho, Okinawa 903-0213, Japan
Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru 1, Nishihara-cho, Okinawa 903-0219, Japan
* Author to whom correspondence should be addressed.
Received: 2 May 2012; in revised form: 17 May 2012 / Accepted: 18 May 2012 / Published: 25 May 2012
Abstract: Oxidation of low-density lipoprotein (LDL) is the principal risk factor for the development of atherosclerosis. In this study, we used several methods to investigate the ability of the acetone extract from rhizomes, stems, leaves, flowers, pericarps and seeds of Alpinia zerumbet to inhibit atherosclerosis in vitro. The seed extract had the strongest activity against tyrosinase, pancreatic lipase (PL), 15-lipoxygenase (15-LO) and LDL oxidation activities (IC50 = 2.30 ± 0.02, 5.00 ± 0.07, 1.29 ± 0.07 and 15.40 ± 0.86 µg/mL, respectively), amongst all different parts. It also had similar effects to the positive controls. Most of the extracts showed partial agonistic properties towards estrogenic activity. Cholest-4-ene-3,6-dione, a steroid present only in the seed extract seems to be the compound responsible for these activities. The results showed that cholest-4-ene-3,6-dione had similar ability to curcumin and quercetin against PL and LDL oxidation (IC50 = 19.50 ± 1.17 and 16.12 ± 1.43 µg/mL, respectively). Furthermore, cholest-4-ene-3,6-dione (IC50 = 34.21 ± 1.31 µg/mL) had higher inhibition against 15-LO than quercetin (IC50 = 54.79 ± 1.12 µg/mL).
Keywords: Alpinia zerumbet; acetone extracts; atherosclerosis; LDL oxidation; cholest-4-ene-3,6-dione
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Chompoo, J.; Upadhyay, A.; Gima, S.; Fukuta, M.; Tawata, S. Antiatherogenic Properties of Acetone Extract of Alpinia zerumbet Seeds. Molecules 2012, 17, 6237-6248.
Chompoo J, Upadhyay A, Gima S, Fukuta M, Tawata S. Antiatherogenic Properties of Acetone Extract of Alpinia zerumbet Seeds. Molecules. 2012; 17(6):6237-6248.
Chompoo, Jamnian; Upadhyay, Atul; Gima, Shinichi; Fukuta, Masakazu; Tawata, Shinkichi. 2012. "Antiatherogenic Properties of Acetone Extract of Alpinia zerumbet Seeds." Molecules 17, no. 6: 6237-6248.