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Assessment of Worldwide Genetic Diversity of Siberian Wild Rye (Elymus sibiricus L.) Germplasm Based on Gliadin Analysis
Department of Grassland Science, Sichuan Agricultural University, Yaan 625014, China
Sichuan Academy of Grassland Science, Chengdu 611731, China
* Author to whom correspondence should be addressed.
Received: 6 February 2012; in revised form: 9 March 2012 / Accepted: 26 March 2012 / Published: 12 April 2012
Abstract: E. sibiricus L., the type species of the genus Elymus, is a perennial, self-pollinating and allotetraploid grass indigenous to Northern Asia, which in some countries can be cultivated as an important forage grass. In the present study, eighty-six Elymus sibiricus accessions, mostly from different parts of Asia, were assayed by gliadin markers based on Acid Polyacrylamide Gel Electrophoresis to differentiate and explore their genetic relationships. The genetic similarity matrix was calculated by 47 polymorphic bands, which ranged from 0.108 to 0.952 with an average of 0.373. The total Shannon diversity index (Ho) and the Simpson index (He) was 0.460 and 0.302, respectively. Cluster analysis showed a clear demarcation between accessions from Qinghai-Tibetan Plateau, China and the others as separate groups. The clustering pattern was probably dependent on geographic origin and ecological adaptability of the accessions. The population structure analysis based on Shannon indices showed that the proportion of variance within and among the five geographic regions of the Northern Hemisphere was 55.9 and 44.1%, respectively, or 63.4 and 36.6% within and among six Chinese provinces. This distinct geographical divergence was perhaps depended on ecogeographical conditions such as climate difference and mountain distribution. The results of gladin analysis in this study are useful for the collection and preservation of E. sibiricus germplasm resources.
Keywords: Elymus sibiricus; genetic diversity; cluster analysis; gladin markers
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MDPI and ACS Style
Ma, X.; Chen, S.; Zhang, X.; Bai, S.; Zhang, C. Assessment of Worldwide Genetic Diversity of Siberian Wild Rye (Elymus sibiricus L.) Germplasm Based on Gliadin Analysis. Molecules 2012, 17, 4424-4434.
Ma X, Chen S, Zhang X, Bai S, Zhang C. Assessment of Worldwide Genetic Diversity of Siberian Wild Rye (Elymus sibiricus L.) Germplasm Based on Gliadin Analysis. Molecules. 2012; 17(4):4424-4434.
Ma, Xiao; Chen, Shiyong; Zhang, Xinquan; Bai, Shiqie; Zhang, Changbing. 2012. "Assessment of Worldwide Genetic Diversity of Siberian Wild Rye (Elymus sibiricus L.) Germplasm Based on Gliadin Analysis." Molecules 17, no. 4: 4424-4434.