Inhibition of Melanogenesis Versus Antioxidant Properties of Essential Oil Extracted from Leaves of Vitex negundo Linn and Chemical Composition Analysis by GC-MS
Abstract
:1. Introduction
2. Results and Discussion
2.1. Inhibition of Melanogenesis by V. negundo Linn Essential Oil
2.1.1. Inhibitory Effect of V. negundo Linn Essential Oil on Mushroom Tyrosinase Activity
2.1.2. Effect of V. negundo Linn Essential Oil on Melanin Synthesis in B16F10 Cells
2.1.3. Effects of V. negundo Linn Essential Oil on Tyrosinase Activity in B16F10 Cells
2.2. Antioxidant Capacity of V. negundo Linn Essential Oil
2.2.1. DPPH Assay
2.2.2. ABTS Assay
2.2.3. Determination of Reducing Power of V. negundo Linn Essential Oil
2.2.4. Metal-Ion Chelating Activity of V. negundo Linn Essential Oil
2.3. Chemical Composition of V. negundo Linn Essential Oil
Rta | Compound b | M. f. c | Peak area (%) | Classification |
---|---|---|---|---|
23.99 | Sabinene | C10H16 | 19.04 | monoterpene |
26.80 | Eucalyptol | C10H17O | 0.40 | ether |
27.25 | o-Cymene | C10H14 | 5.90 | aromatic compound |
27.47 | β-Terpinyl acetate | C12H20O2 | 8.99 | ester |
28.18 | 1R- α-Pinene | C10H16 | 0.21 | monoterpene |
30.94 | Linalool | C10H18O | 4.27 | alcohol |
33.03 | Menthone | C10H18O | 4.96 | ketone |
33.97 | (−)-Menthol | C10H19O | 1.44 | alcohol |
34.15 | (−)-Terpinen-4-ol | C10H19O | 2.82 | alcohol |
39.07 | Linalyl formate | C11H18O2 | 3.72 | ester |
46.54 | Nerol acetate | C12H20O2 | 1.18 | ester |
47.46 | Geranyl acetate | C11H20O2 | 0.88 | ester |
48.83 | Caryophyllene | C15H24 | 18.27 | Sesquiterpene |
49.54 | α-Bergamotene | C15H24 | 0.53 | Sesquiterpene |
50.13 | Humulene | C15H24 | 0.58 | Sesquiterpene |
51.37 | Eremophilene | C15H24 | 12.76 | Sesquiterpene |
52.11 | β-Bisabolene | C15H24 | 0.94 | Sesquiterpene |
54.31 | Caryophyllene oxide | C15H24O | 11.33 | Sesquiterpene |
Unknown | 1.78 |
3. Experimental
3.1. Plant Material and Isolation of Essential Oils
3.2. Mushroom Tyrosinase Actvity Assay
3.3. Intracellular Melanin Content Measurement
3.4. Intracellular Tyrosinase Activity Assay
3.5. DPPH Scavenging Activity Assay
3.6. ABTS+ Scavenging Capacity Assay
3.7. Determination of Reducing Power
3.8. Measurement of Metal-Ion Chelating Capacity
3.9. Gas Chromatography-Mass Spectrometry (GC-MS)
3.10. Statistical Analysis
4. Conclusions
Acknowledgements
References and Notes
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Huang, H.-C.; Chang, T.-Y.; Chang, L.-Z.; Wang, H.-F.; Yih, K.-H.; Hsieh, W.-Y.; Chang, T.-M. Inhibition of Melanogenesis Versus Antioxidant Properties of Essential Oil Extracted from Leaves of Vitex negundo Linn and Chemical Composition Analysis by GC-MS. Molecules 2012, 17, 3902-3916. https://doi.org/10.3390/molecules17043902
Huang H-C, Chang T-Y, Chang L-Z, Wang H-F, Yih K-H, Hsieh W-Y, Chang T-M. Inhibition of Melanogenesis Versus Antioxidant Properties of Essential Oil Extracted from Leaves of Vitex negundo Linn and Chemical Composition Analysis by GC-MS. Molecules. 2012; 17(4):3902-3916. https://doi.org/10.3390/molecules17043902
Chicago/Turabian StyleHuang, Huey-Chun, Tzu-Yun Chang, Long-Zen Chang, Hsiao-Fen Wang, Kuang-Hway Yih, Wan-Yu Hsieh, and Tsong-Min Chang. 2012. "Inhibition of Melanogenesis Versus Antioxidant Properties of Essential Oil Extracted from Leaves of Vitex negundo Linn and Chemical Composition Analysis by GC-MS" Molecules 17, no. 4: 3902-3916. https://doi.org/10.3390/molecules17043902