Molecules 2012, 17(4), 3917-3932; doi:10.3390/molecules17043917
Article

Antioxidant, Antimicrobial Properties and Phenolics of Different Solvent Extracts from Bark, Leaves and Seeds of Pongamia pinnata (L.) Pierre

Received: 29 January 2012; in revised form: 17 March 2012 / Accepted: 21 March 2012 / Published: 30 March 2012
(This article belongs to the Section Natural Products)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: This study appraises the antioxidant and antimicrobial attributes of various solvent extracts (absolute methanol, aqueous methanol, absolute ethanol, aqueous ethanol, absolute acetone, aqueous acetone, and deionized water) from bark, leaves and seeds of Pongamia pinnata (L.) Pierre. Maximum extraction yield of antioxidant components from bark (16.31%), leaves (11.42%) and seeds (21.51%) of P. pinnata was obtained using aqueous methanol (20:80). Of the extracts tested, the bark extract, obtained with aqueous methanol, exhibited greater levels of total phenolics [6.94 g GAE/100 g dry weight (DW)], total flavonoids (3.44 g CE/100 g DW), inhibition of linoleic acid peroxidation (69.23%) and DPPH radical scavenging activity (IC50 value, 3.21 μg/mL), followed by leaves and seeds extracts. Bark extract tested against a set of bacterial and fungal strains also revealed the strongest antimicrobial activity with the largest inhibition zone and lowest minimum inhibitory concentration (MIC). HPLC analysis of aqueous methanol extracts from bark, leaves and seeds indicated the presence of protocatechuic, ellagic, ferulic, gallic, gentisic, 4-hydroxybenzoic and 4-hydroxycinnamic acids in bark (1.50–6.70 mg/100 g DW); sorbic, ferulic, gallic, salicylic and p-coumaric acids in leaves (1.18–4.71 mg/100 g DW); vanillic, gallic and tannic acids in seeds (0.52–0.65 mg/100 g DW) as the main phenolic acids. The present investigation concludes that the tested parts of P. pinnata, in particular the bark, have strong potential for the isolation of antioxidant and antimicrobial agents for functional food and pharmaceutical uses.
Keywords: P. pinnata; solvent extracts; antioxidants; antimicrobial; HPLC; phenolic acids
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MDPI and ACS Style

Sajid, Z.I.; Anwar, F.; Shabir, G.; Rasul, G.; Alkharfy, K.M.; Gilani, A.-H. Antioxidant, Antimicrobial Properties and Phenolics of Different Solvent Extracts from Bark, Leaves and Seeds of Pongamia pinnata (L.) Pierre. Molecules 2012, 17, 3917-3932.

AMA Style

Sajid ZI, Anwar F, Shabir G, Rasul G, Alkharfy KM, Gilani A-H. Antioxidant, Antimicrobial Properties and Phenolics of Different Solvent Extracts from Bark, Leaves and Seeds of Pongamia pinnata (L.) Pierre. Molecules. 2012; 17(4):3917-3932.

Chicago/Turabian Style

Sajid, Zahid Iqbal; Anwar, Farooq; Shabir, Ghulam; Rasul, Ghulam; Alkharfy, Khalid M.; Gilani, Anwarul-Hassan. 2012. "Antioxidant, Antimicrobial Properties and Phenolics of Different Solvent Extracts from Bark, Leaves and Seeds of Pongamia pinnata (L.) Pierre." Molecules 17, no. 4: 3917-3932.

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