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Molecules 2012, 17(4), 3890-3901; doi:10.3390/molecules17043890

Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases

1
Department of Dental Hygiene, College of Natural Sciences, Dongeui University, Busan 614-714, Korea
2
Department of Dental Hygiene, Ulsan College, San 160-1, Hwajeong-Dong, Dong-Gu, Ulsan 682-715, Korea
*
Author to whom correspondence should be addressed.
Received: 5 March 2012 / Revised: 5 March 2012 / Accepted: 20 March 2012 / Published: 30 March 2012
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Abstract

Cryptomeria japonica D. Don (C. japonica) has been used in traditional medicines from Asia for a variety of indications, including liver ailments, and an antitussive, and for its antiulcer activities. We examined the cell viability and apoptosis of KB cells treated with C. japonica essential oil at several concentrations for 12 h by MTT assay, Hoechst-33258 dye staining, DNA fragmentation, flow cytometry (cell cycle), and Western blotting for mitochondria stress, activation of caspases, and poly (ADP-ribose) polymerase. The essential oil induced the apoptosis of KB cells in a dose-dependent manner, which was verified by DNA fragmentation, appearance of apoptotic bodies, and the sub-G1 ratio. The essential oil also induced rapid and transient caspase-3 activity and cleavage of PARP of the KB cells. Treating the cells with the oil also caused changes in the mitochondrial level of the Bcl-2 family proteins such as Bcl-2 and Bax, thereby inducing the release of cytochrome c into the cytosol. The essential oil of C. japonica may have potential as a cancer chemopreventive and therapeutic agent.
Keywords: Cryptomeria japonica; essential oil; apoptosis; mitochondrial stress; caspases Cryptomeria japonica; essential oil; apoptosis; mitochondrial stress; caspases
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Cha, J.-D.; Kim, J.-Y. Essential Oil from Cryptomeria japonica Induces Apoptosis in Human Oral Epidermoid Carcinoma Cells via Mitochondrial Stress and Activation of Caspases. Molecules 2012, 17, 3890-3901.

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