• Abstract
• Full-Text PDF [228 KB]
• Full-Text XML
• Article Versions Notes

• Article Statistics
• PubMed/Medline
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Li, Y
• Shan, T
• Mou, Y
• Li, P
• Zhao, J
• Zhao, W
• Peng, Y
• Zhou, L
• Ding, C
• [+] on Google Scholar
• Li, Y
• Shan, T
• Mou, Y
• Li, P
• Zhao, J
• Zhao, W
• Peng, Y
• Zhou, L
• Ding, C
• [+] on PubMed
• Li, Y
• Shan, T
• Mou, Y
• Li, P
• Zhao, J
• Zhao, W
• Peng, Y
• Zhou, L
• Ding, C

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Molecules 2012, 17(4), 3761-3773; doi:10.3390/molecules17043761

Article

Enhancement of Palmarumycin C₁₂ and C₁₃ Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis

Yan Li ^1,2 , Tijiang Shan ² , Yan Mou ² , Peiqin Li ² , Jianglin Zhao ² , Wensheng Zhao ² , Youliang Peng ² , Ligang Zhou ^2,*  and Chunbang Ding ^1,*

¹ College of Biology and Science, Sichuan Agricultural University, Yaan 625014, China ² College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China

* Authors to whom correspondence should be addressed.

Received: 19 January 2012; in revised form: 21 March 2012 / Accepted: 22 March 2012 / Published: 26 March 2012

(This article belongs to the Special Issue Natural Polysaccharides: Chemistry, Bioactivity and Analysis)

Download PDF Full-Text [228 KB, uploaded 26 March 2012 11:14 CEST]

Abstract: Three crude oligosaccharides were respectively prepared by acid hydrolysis of three polysaccharides, which were water-extracted polysaccharide (WEP), sodium hydroxide-extracted polysaccharide (SEP) and acid-extracted polysaccharide (AEP) from the rhizomes of Dioscorea zingiberensis. Among the three oligosaccharides, the crude oligosaccharide prepared by acid hydrolysis of WEP was found to be the most efficient elicitor to enhance the production of palmarumycins C₁₂ and C₁₃ in liquid culture of endophytic fungus Berkleasmium sp. Dzf12. When OW was applied to the medium at 300 mg/L on day 3 of culture, the maximal yields of palmarumycin C₁₂ (87.96 mg/L) and palmarumycin C₁₃ (422.28 mg/L) were achieved on day 15 of culture, which were 9.83 and 3.24-fold in comparison with those (8.95 and 130.43 mg/L) of control, respectively. The results indicate that addition of the oligosaccharides from the host plant D. zingiberensis should be an effective strategy for enhancing production of palmarumycins C₁₂ and C₁₃ in liquid culture of endophytic fungus Berkleasmium sp. Dzf12.

Keywords: endophytic fungus; Berkleasmium sp. Dzf12; spirobisnaphthalene; palmarumycin C₁₂; palmarumycin C₁₃; crude oligosaccharide; polysaccharide; elicitation; Dioscorea zingiberensis

Article Statistics

Cite This Article