MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. MDP Induces the Gene Expression of Type I Interferons
2.2. The Effect of MDP on the Gene Expression of NOD1 and NOD2
2.3. The Up-Regulation of Interferon Gene Expression is TNFα-independent
2.4. MDP Induces the Phosphorylation of IFR3 in HAECs
2.5. MDP Induces the Gene Expression of Pro-Inflammatory Cytokines
3. Experimental
3.1. Cell Lines and Reagents
3.2. Reverse Transcription-PCR (RT-PCR)
GAPDH-F, | AATCCCATCACCATCTTCCA, | GAPDH-R, | CCTGCTTCACCACCTTCTTG; |
IFNα-F, | AGCTGCAAGTCAAGCTGCTCT, | IFNα-R, | TTCTTCACAGCCAAGATGGA; |
IFNβ-F, | TCTCCTCCAAATTGCTCTCCT, | IFNβ-R, | TACTCCTTGGCCTTCAGGTAA; |
IL-1β-F, | TTGAAGCTGATGGCCCTAAAC, | IL-1β-R, | CACCAAGCTTTTTTGCTGTG; |
IL-6-F, | CCAGTACCCCCAGGAGAAGAT, | IL-6-R, | TTGCCTTTTTCTGCAGGAAC; |
IL-8-F, | TTGGCAGCCTTCCTGATTT, | IL-8-R, | TCAAAAACTTCTCCACAACCC; |
IRF1-F, | GAGATGATCTTCCAGATCCCA, | IRF1-A, | TGTAGCTGCTGTGGTCATCA; |
IRF2-F, | GGTGGGATGTGGAAAAAGATG, | IRF2-A, | AAATGTCTGGCGGATTGGT; |
IRF3-F, | AACCTGGAAGAGGAATTTCCG, | IRF3-A, | ATGGTCTGCTGGAAGACTTGG; |
IRF4-F, | TGTGGGAGAACGAGGAGAAGA, | IRF4-A, | TCCAAACGTCATGGGACATT; |
IRF5-F, | GGTCAACGGGGAAAAGAAA, | IRF5-A, | CCTGCACCAAAAGAGTAATCC; |
IRF6-F, | TAAACGCTTCCAGATTCCCT, | IRF6-A, | TGATCCAGCTCATCTTCCTCA; |
IRF7-F, | TGGCTCCTTGGAGAGATCA, | IRF7-A, | TTGGAGTCCAGCATGTGTGT; |
IRF8-F, | ACGCTGGCAAGCAAGATTAT, | IRF8-A, | TCTGGGAGAATGCTGAATGGT; |
IRF9-F, | CAGGATGCTGCCTTCTTCAA, | IRF9-A, | TGCTGCTCCCAATGTCTGAAT; |
TNFα-F, | TGACAAGCCTGTAGCCCATGTT, | TNFα-R, | AGGGCAATGATCCCAAAGTAGA |
3.3. Immunoblot
3.4. Quantitative Real Time RT-PCR (qRT-PCR)
3.5. Statistical Analysis
4. Conclusions
Acknowledgments
Conflict of Interest
- Sample Availability: Samples of the compounds are commercially available.
References and Notes
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Lv, Q.; Yang, M.; Liu, X.; Zhou, L.; Xiao, Z.; Chen, X.; Chen, M.; Xie, X.; Hu, J. MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells. Molecules 2012, 17, 3599-3608. https://doi.org/10.3390/molecules17043599
Lv Q, Yang M, Liu X, Zhou L, Xiao Z, Chen X, Chen M, Xie X, Hu J. MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells. Molecules. 2012; 17(4):3599-3608. https://doi.org/10.3390/molecules17043599
Chicago/Turabian StyleLv, Qingshan, Mei Yang, Xueting Liu, Lina Zhou, Zhilin Xiao, Xiaobin Chen, Meifang Chen, Xiumei Xie, and Jinyue Hu. 2012. "MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells" Molecules 17, no. 4: 3599-3608. https://doi.org/10.3390/molecules17043599