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Molecules 2012, 17(3), 3586-3598; doi:10.3390/molecules17033586

Effects of a Natural Prolyl Oligopeptidase Inhibitor, Rosmarinic Acid, on Lipopolysaccharide-Induced Acute Lung Injury in Mice

1,2,†, 2,†, 1,†, 3, 2, 4, 2, 2, 2,*  and 1,*
1 College of Animal Science and Technology, Guangxi University, 100 Daxue Road, Nanning 530005, Guangxi, China 2 Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China 3 Department of Dermatology, Second Hospital of Jilin University, Changchun 130041, Jilin, China 4 College of Plant Science, Jilin University, Changchun 130062, Jilin, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 11 February 2012 / Revised: 6 March 2012 / Accepted: 9 March 2012 / Published: 22 March 2012
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Rosmarinic acid (RA), a polyphenolic phytochemical, is a natural prolyl oligopeptidase inhibitor. In the present study, we found that RA exerted potent anti-inflammatory effects in in vivo models of acute lung injury (ALI) induced by lipopolysaccharide (LPS). Mice were pretreated with RA one hour before challenge with a dose of 0.5 mg/kg LPS. Twenty-four hours after LPS was given, bronchoalveolar lavage fluid (BALF) was obtained to measure pro-inflammatory mediator and total cell counts. RA significantly decreased the production of LPS-induced TNF-a, IL-6, and IL-1β compare with the LPS group. When pretreated with RA (5, 10, or 20 mg/kg) the lung wet-to-dry weight (W/D) ratio of the lung tissue and the number of total cells, neutrophils and macrophages in the BALF were decreased significantly. Furthermore, RA may enhance oxidase dimutase (SOD) activity during the inflammatory response to LPS-induced ALI. And we further demonstrated that RA exerts anti-inflammation effect in vivo models of ALI through suppresses ERK/MAPK signaling in a dose dependent manner. These studies have important implications for RA administration as a potential treatment for ALI.
Keywords: rosmarinic acid; LPS; SOD; MAPK; ALI rosmarinic acid; LPS; SOD; MAPK; ALI
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chu, X.; Ci, X.; He, J.; Jiang, L.; Wei, M.; Cao, Q.; Guan, M.; Xie, X.; Deng, X.; He, J. Effects of a Natural Prolyl Oligopeptidase Inhibitor, Rosmarinic Acid, on Lipopolysaccharide-Induced Acute Lung Injury in Mice. Molecules 2012, 17, 3586-3598.

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