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Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts
Institute for Medicinal Plant Research “Dr Josif Pančić”, Department of Pharmaceutical Research and Development, Tadeuša Košćuška 1, Belgrade 11 000, Serbia
ICN Montenegro, Mitra Bakića 64, Podgorica 20 000, Montenegro
Faculty of Veterinary Medicine, Department of Microbiology and Immunology, Univesity of Belgade, Bulevar oslobođenja 18, Belgrade 11 000, Serbia
Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade Karnegijeva 4, P.O. Box 3503, Belgrade 11 120, Serbia
Faculty of Medicine, Department of Pharmacology, University of Kragujevac, Slobodana Markovića 69, Kragujevac 34 000, Serbia
* Author to whom correspondence should be addressed.
Received: 16 January 2012; in revised form: 22 February 2012 / Accepted: 28 February 2012 / Published: 5 March 2012
(This article belongs to the Special Issue Terpenoids
Abstract: Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 °C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 μg/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.
Keywords: Sideritis scardica Griseb.; Lamiaceae (mountain tea); essential oil; hydrodistillation; supercritical carbon dioxide extracts; solvent extraction; terpenoids; antimicrobial activity; GC and GC-MS analysis
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Tadić, V.; Bojović, D.; Arsić, I.; Đorđević, S.; Aksentijevic, K.; Stamenić, M.; Janković, S. Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. Molecules 2012, 17, 2683-2703.
Tadić V, Bojović D, Arsić I, Đorđević S, Aksentijevic K, Stamenić M, Janković S. Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. Molecules. 2012; 17(3):2683-2703.
Tadić, Vanja; Bojović, Dragica; Arsić, Ivana; Đorđević, Sofija; Aksentijevic, Ksenija; Stamenić, Marko; Janković, Slobodan. 2012. "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts." Molecules 17, no. 3: 2683-2703.