Abstract: To elucidate the molecular mechanisms involved in the therapeutic effects of proanthocyanidins from grape seeds (GSPE), we explore whether GSPE regulates the inflammatory response of TNBS-induced colitis in rats at the levels of NF-κB signal transduction pathway. Rats were intragastrically administered of different doses of GSPE (100, 200 and 400 mg·kg−1) per day for seven days after ulcerative colitis (UC) was induced by intracolonic injection of 2,4,6-trinitrobenzenesulfonic acid (TNBS) dissolved in 50% ethanol. Sulfasalazine (SASP) at 400 mg/kg was used as a positive control drug. The expression of nuclear factor-kappa B (NF-κB), phospho-I kappaB-alpha (pIκBα), inhibitor kappa B kinase (IκK) in the colon tissues were all measured by enzyme-linked immunosorbent assay (ELISA) methods. Treatment with GSPE reduced the expression of NF-κB, pIκBα and IκK in the colon. The results of this study show that GSPE exerts beneficial effects in inflammatory bowel disease by inhibition of NF-κB signal transduction pathways.
Keywords: proanthocyanidins from grape seeds; ulcerative colitis; nuclear factor-kappa B
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Li, X.; Yang, X.; Cai, Y.; Qin, H.; Wang, L.; Wang, Y.; Huang, Y.; Wang, X.; Yan, S.; Wang, L.; Zhao, X.; Li, W.; Li, S.; Chen, J.; Wu, Y. Proanthocyanidins from Grape Seeds Modulate the NF-κB Signal Transduction Pathways in Rats with TNBS-Induced Ulcerative Colitis. Molecules 2011, 16, 6721-6731.
Li X, Yang X, Cai Y, Qin H, Wang L, Wang Y, Huang Y, Wang X, Yan S, Wang L, Zhao X, Li W, Li S, Chen J, Wu Y. Proanthocyanidins from Grape Seeds Modulate the NF-κB Signal Transduction Pathways in Rats with TNBS-Induced Ulcerative Colitis. Molecules. 2011; 16(8):6721-6731.
Li, Xiaoli; Yang, Xiaolai; Cai, Yongqing; Qin, Hong; Wang, Li; Wang, Yanhong; Huang, Yanhui; Wang, Xiaoxia; Yan, Shuai; Wang, Liping; Zhao, Xin; Li, Wan; Li, Sijia; Chen, Jiajia; Wu, Yongjie. 2011. "Proanthocyanidins from Grape Seeds Modulate the NF-κB Signal Transduction Pathways in Rats with TNBS-Induced Ulcerative Colitis." Molecules 16, no. 8: 6721-6731.