Alkylphenol Activity against Candida spp. and Microsporum canis: A Focus on the Antifungal Activity of Thymol, Eugenol and O-Methyl Derivatives
Abstract
:1. Introduction
2. Results and Discussion
Growth Inhibition Zones (mm) | ||||||||
Alkylphenols (10,000 µg/mL) | Controls | |||||||
Strains | thymol | methyl-thymol | eugenol | methyl-eugenol | anethole | estragole | griseofulvin (1000 µg/mL) | amphotericin B (5 µg/mL) |
M. canis | ||||||||
CEMM 01-3-188 | 35 | 22 | 26 | 20 | 16 | 23 | 55 | - |
CEMM 01-5-190 | 40 | 20 | 30 | 20 | 20 | 20 | 47 | - |
C. albicans | ||||||||
CEMM 01-3-075 | 18 | 18 | 12 | 10 | 11 | 11 | - | 14 |
CEMM 01-3-069 | 17 | 13 | 8 | 7 | 9 | 8 | - | 10 |
Alkylphenols (µg/mL) | Controls (μg/mL) | |||||||||||||
Strains | thymol | methyl-thymol | eugenol | methyl-eugenol | anethole | estragole | amphotericin B | griseofulvin | ||||||
MIC | MFC | MIC | MFC | MIC | MFC | MIC | MFC | MIC | MFC | MIC | MFC | MIC | MIC | |
C. albicans (n = 4) | 39 (4)a | 78 (4) | 1250 (4) | 2500 (4) | 620 (3) | 1250 (3) | 620 (3) | 1250 (3) | 620 (4) | 1250 (4) | 1250 (3) | 2500 (3) | 0.5 (2) | - |
150 (1) | 310 (1) | 310 (1) | 620 (1) | 620 (1) | 1250 (1) | 0.25 (2) | ||||||||
C. tropicalis (n = 2) | 39 (2) | 78 (2) | 1250 (1) | 2500 (1) | 310 (2) | 620 (2) | 310 (2) | 620 (2) | 620 (2) | 1250 (2) | 620 (2) | 1250 (2) | 1.0 (1) | - |
620 (1) | 1250 (1) | 0.5 (1) | ||||||||||||
C. krusei (n = 1) | 150 | 310 | 1250 | 2500 | 620 | 1250 | 620 | 1250 | 620 | 1250 | 620 | 1250 | 0.5 | - |
ATCC 22019 | ||||||||||||||
C. parapsiloses (n = 1) | 150 | 310 | 1250 | 2500 | 620 | 1250 | 620 | 1250 | 620 | 1250 | 620 | 1250 | 2.0 | - |
ATCC 6528 | ||||||||||||||
M. canis (n = 6) | 9.7 (3) | 19 (3) | 150 (4) | 310 (4) | 39 (6) | 78 (6) | 150 (2) | 310 (2) | 150 (3) | 310 (3) | 39 (4) | 78 (4) | - | 0.25 (2) |
4.8 (3) | 9.7 (3) | 78 (2) | 150 (2) | 78 (4) | 150 (4) | 78 (3) | 150 (3) | 19 (2) | 39 (2) | 0.125 (3) | ||||
0.006 (1) |
3. Experimental
3.1. General
3.2. Fungal strains
3.3. Agar-well diffusion method
3.4. Broth microdilution method
3.5. Statistical analysis
4. Conclusions
Acknowledgements
References
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Fontenelle, R.O.S.; Morais, S.M.; Brito, E.H.S.; Brilhante, R.S.N.; Cordeiro, R.A.; Lima, Y.C.; Brasil, N.V.G.P.S.; Monteiro, A.J.; Sidrim, J.J.C.; Rocha, M.F.G. Alkylphenol Activity against Candida spp. and Microsporum canis: A Focus on the Antifungal Activity of Thymol, Eugenol and O-Methyl Derivatives. Molecules 2011, 16, 6422-6431. https://doi.org/10.3390/molecules16086422
Fontenelle ROS, Morais SM, Brito EHS, Brilhante RSN, Cordeiro RA, Lima YC, Brasil NVGPS, Monteiro AJ, Sidrim JJC, Rocha MFG. Alkylphenol Activity against Candida spp. and Microsporum canis: A Focus on the Antifungal Activity of Thymol, Eugenol and O-Methyl Derivatives. Molecules. 2011; 16(8):6422-6431. https://doi.org/10.3390/molecules16086422
Chicago/Turabian StyleFontenelle, Raquel O.S., Selene M. Morais, Erika H.S. Brito, Raimunda S.N. Brilhante, Rossana A. Cordeiro, Ynayara C. Lima, Nilce V.G.P.S. Brasil, André J. Monteiro, José J.C. Sidrim, and Marcos F.G. Rocha. 2011. "Alkylphenol Activity against Candida spp. and Microsporum canis: A Focus on the Antifungal Activity of Thymol, Eugenol and O-Methyl Derivatives" Molecules 16, no. 8: 6422-6431. https://doi.org/10.3390/molecules16086422