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Antioxidant Activities of Extracts and Fractions from Eupatorium lindleyanum DC
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China
* Author to whom correspondence should be addressed.
Received: 31 May 2011; in revised form: 12 July 2011 / Accepted: 15 July 2011 / Published: 19 July 2011
Abstract: The antioxidant activities of water extract (WE), ethanol extract (EE), residue water extract (RWE) and petroleum ether (PF), ethyl acetate (EF), n-BuOH (BF) and water (WF) fractions of the ethanol extract from Eupatorium Lindley DC were investigated for the first time. Total phenolics content, DPPH radical scavenging activities, superoxide radical scavenging activities, total reduction capability, and ferrous ions chelating activities were determined for all the extracts and fractions. The results showed that all the extracts and fractions exhibited antioxidant activities with different magnitudes of potency. Among all the samples, WE and RWE exhibited the best antioxidant capacities, the BF also exhibited high antioxidant abilities in all tests except for the metal chelating activity, while the other extracts and fractions were relatively weak antioxidants. The BF had the highest total phenolics contents in all extracts and fractions, and the WE and RWE were found to be rich in tannins. Furthermore, the content of total phenolics showed good correlation with DPPH radical scavenging activity, superoxide anion radical scavenging activity, and the reducing power. Phenolic composition of all the extracts and fractions was identified and quantified by HPLC. The results indicate that the extracts of E. Lindley DC might be a useful potential source of natural antioxidant ingredients.
Keywords: Eupatorium lindleyanum DC; antioxidant activity; extracts; phenolics
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Yan, G.; Ji, L.; Luo, Y.; Hu, Y. Antioxidant Activities of Extracts and Fractions from Eupatorium lindleyanum DC. Molecules 2011, 16, 5998-6009.
Yan G, Ji L, Luo Y, Hu Y. Antioxidant Activities of Extracts and Fractions from Eupatorium lindleyanum DC. Molecules. 2011; 16(7):5998-6009.
Yan, Guilong; Ji, Lilian; Luo, Yuming; Hu, Yonghong. 2011. "Antioxidant Activities of Extracts and Fractions from Eupatorium lindleyanum DC." Molecules 16, no. 7: 5998-6009.